Exploring the mechanism of IgA vasculitis pathogenesis through the interaction of thrombin and inflammatory factors using urinary proteomics
10.7499/j.issn.1008-8830.2311151
- VernacularTitle:运用尿蛋白组学技术探索凝血酶与炎症因子相互作用参与IgA血管炎发病的机制
- Author:
Meng-Meng LIU
1
,
2
;
Gai-Ling HOU
;
Xiao-Qing YANG
;
Qiu-Shuang ZHANG
;
Xiao-Feng MEI
;
Ying DING
;
Lan SONG
;
Yan-Jie HUANG
Author Information
1. 河南中医药大学儿科医学院,河南郑州 450046
2. 河南中医药大学第一附属医院儿科医院,河南郑州 450046
- Keywords:
IgA vasculitis;
Proteomics;
Thrombin;
Hypercoagulability;
Inflammation;
Child
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(7):683-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the evidence,urinary biomarkers,and partial mechanisms of hypercoagulability in the pathogenesis of IgA vasculitis(IgAV).Methods Differential expression of proteins in the urine of 10 healthy children and 10 children with IgAV was screened using high-performance liquid chromatography-tandem mass spectrometry,followed by Reactome pathway analysis.Protein-protein interaction(PPI)network analysis was conducted using STRING and Cytoscape software.In the validation cohort,15 healthy children and 25 children with IgAV were included,and the expression levels of differential urinary proteins were verified using enzyme-linked immunosorbent assay.Results A total of 772 differential proteins were identified between the IgAV group and the control group,with 768 upregulated and 4 downregulated.Reactome pathway enrichment results showed that neutrophil degranulation,platelet activation,and hemostasis pathways were involved in the pathogenesis of IgAV.Among the differential proteins,macrophage migration inhibitory factor(MIF)played a significant role in neutrophil degranulation and hemostasis,while thrombin was a key protein in platelet activation and hemostasis pathways.PPI analysis indicated that thrombin directly interacted with several proteins involved in inflammatory responses,and these interactions involved MIF.Validation results showed that compared to healthy children,children with IgAV had significantly higher urine thrombin/creatinine and urine MIF/creatinine levels(P<0.05).Conclusions Thrombin contributes to the pathogenesis of IgAV through interactions with inflammatory factors.Urinary thrombin and MIF can serve as biomarkers reflecting the hypercoagulable and inflammatory states in children with IgAV.
