Characteristics of drug resistance and biofilm formation in carbapenem-resistant Acinetobacter baumannii in hospitalized children
10.7499/j.issn.1008-8830.2309058
- VernacularTitle:住院患儿耐碳青霉烯类鲍曼不动杆菌的耐药及生物膜特征分析
- Author:
Yun-Yun LI
1
;
Hou-Chang LIU
;
Hai-Ping WANG
;
Ting-Yi DU
;
Li JIANG
Author Information
1. 昆明市儿童医院/昆明医科大学附属儿童医院检验科,云南昆明 650100
- Keywords:
Acinetobacter baumannii;
Carbapenemase;
Biofilm;
Child
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(4):358-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the distribution,drug resistance,and biofilm characteristics of carbapenem-resistant Acinetobacter baumannii(CRAB)isolated from hospitalized children,providing a reference for the prevention and treatment of CRAB infections in hospitalized children.Methods Forty-eight CRAB strains isolated from January 2019 to December 2022 were classified into epidemic and sporadic strains using repetitive extragenic palindromic sequence-based polymerase chain reaction.The drug resistance,biofilm phenotypes,and gene carriage of these two types of strains were compared.Results Both the 22 epidemic strains and the 26 sporadic strains were producers of Class D carbapenemases or extended-spectrum β-lactamases with downregulated outer membrane porins,harboring the VIM,OXA-23,and OXA-51 genes.The biofilm formation capability of the sporadic strains was stronger than that of the epidemic strains(P＜0.05).Genes related to biofilm formation,including Bap,bfs,OmpA,CsuE,and intI1,were detected in both epidemic and sporadic strains,with a higher detection rate of the intI1 gene in epidemic strains(P＜0.05).Conclusions CRAB strains are colonized in the hospital,with sporadic strains having a stronger ability to form biofilms,suggesting the potential for forming new clonal transmissions in the hospital.Continuous monitoring of the epidemic trends of CRAB and early warning of the distribution of epidemic strains are necessary to reduce the risk of CRAB infections in hospitalized children.[Chinese Journal of Contemporary Pediatrics,2024,26(4):358-364]
