Specific changes in gut microbiota and short-chain fatty acid levels in infants with cow's milk protein allergy
10.7499/j.issn.1008-8830.2308007
- VernacularTitle:牛奶蛋白过敏婴儿肠道菌群特异性变化及短链脂肪酸水平分析
- Author:
Zhi-Dan YU
1
;
Ling-Ling YUE
;
Zi-Hui WANG
;
Rui-Zi WANG
;
Li-Feng LI
;
Wan-Cun ZHANG
;
Xiao-Qin LI
Author Information
1. 郑州大学附属儿童医院/河南省儿童医院郑州儿童医院消化内科/郑州市儿童消化疾病重点实验室,河南郑州 450018
- Keywords:
Cow's milk protein allergy;
Gut microbiota diversity;
Short-chain fatty acid;
Infant
- From:
Chinese Journal of Contemporary Pediatrics
2024;26(3):236-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes in gut microbiota and levels of short-chain fatty acids(SCFA)in infants with cow's milk protein allergy(CMPA),and to clarify their role in CMPA.Methods A total of 25 infants diagnosed with CMPA at Children's Hospital Affiliated to Zhengzhou University from August 2019 to August 2020 were enrolled as the CMPA group,and 25 healthy infants were selected as the control group.Fecal samples(200 mg)were collected from both groups and subjected to 16S rDNA high-throughput sequencing technology and liquid chromatography-mass spectrometry to analyze the changes in gut microbial composition and metabolites.Microbial diversity was analyzed in conjunction with metabolites.Results Compared to the control group,the CMPA group showed altered gut microbial structure and significantly increased α-diversity(P＜0.001).The abundance of Firmicutes,Clostridiales and Bacteroidetes was significantly decreased,while the abundance of Sphingomonadaceae,Clostridiaceae_l and Mycoplasmataceae was significantly increased in the CMPA group compared to the control group(P＜0.001).Metabolomic analysis revealed reduced levels of acetic acid,butyric acid,and isovaleric acid in the CMPA group compared to the control group,and the levels of the metabolites were positively correlated with the abundance of SCFA-producing bacteria such as Faecalibacterium and Roseburia(P＜0.05).Conclusions CMPA infants have alterations in gut microbial structure,increased microbial diversity,and decreased levels of SCFA,which may contribute to increased intestinal inflammation.[Chinese Journal of Contemporary Pediatrics,2024,26(3):236-243]
