Changes of IGF-1 and its receptor in 3-day-old premature rats with chronic hypoxic-ischemic brain damage
10.3969/j.issn.1008-8830.2005.03.001
- VernacularTitle:3日龄未成熟大鼠慢性缺氧缺血脑损伤IGF-1及其受体的变化
- Author:
HE YING-ZHONG
1
;
Chen CHAO
;
YANG YI
;
CHEN LIAN
;
ZHU LIE-WEI
Author Information
1. 复旦大学附属儿科医院
- Keywords:
Insulin-like growth factor I;
Hypoxia-ischemia,brain;
Rat,premature
- From:
Chinese Journal of Contemporary Pediatrics
2005;7(3):193-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study examined the expression of insulin-like growth factor-1(IGF-1)and its receptor in 3-day-old premature rats with chronic hypoxic ischemic brain damage (HIBD) and investigated the role of IGF-1 in the pathogenesis of this disease.Methods Ninety 3-day-old Sprague-Dawley rats were randomly assigned into a Control group (n =40) and a Hypoxic-ischemic group (HI, n = 50). HI was induced through bilateral common carotid artery ligation. The Control group was only sham-operated. Hematoxylin and eosin staining, TUNEL immunofluorescence staining and immunohistochemistry ways were used to investigate the expression of IGF-1 and its receptor, morphological changes of brain tissues and cell apoptosis of brain white matter. Results The expression of IGF-1 decreased in 3-5 days after HI, but that of its receptor increased in the HI group. The expression changes were most significant at corpus callosum and peri-ventricular white matter and recovered progressively in 7-14 days after HI. After 7 days of HI, the brain white matter presented with morphological changes such as rarefaction, liquefaction and lateral ventricular enlargement. Apoptotic cells in deep white matter increased after HI, and peaked at 48 hrs. Conclusions IGF-1 may play an important role in the pathogenesis of chronic HIBD in 3-day-old premature rats. This study provides an experimental basis for the prevention and treatment of HIBD in premature infants.
