Effects of Moderate Hypothermia on Cerebral Energy Metabolism in Neonatal Rats with Hypoxic-Ischemic Brain Damage
10.3969/j.issn.1008-8830.2003.03.003
- VernacularTitle:亚低温对新生鼠缺氧缺血脑损伤能量代谢的影响
- Author:
YU LI-JUN
1
;
WANG LAI-SHUAN
;
SHAO XIAO-MEI
;
YANG YI
Author Information
1. Children's Hospital of Fudan University
- Keywords:
Moderate hypothermia;
Cerebral hypoxia;
Cerebral ischemia;
Mitochondria;
ATP;
Glucose;
Neonatal rat
- From:
Chinese Journal of Contemporary Pediatrics
2003;5(3):192-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of moderate hypothermia on cerebral glucose and ATP contents andmitochondrial succinate dehydrogenase (SDH) activity in neonatal rats with hypoxic-ischimic brain damage (HIBD).Methods Seven-day-old Sprague-Dawley rat pups were subjected to ligation of the left carotid artery followed by exposureto hypoxia environment of 8 % O2 for 2 h, then they were randomly assigned into normothermic recovered group ( Ingroup) and moderate-hypothermic recovered group (IH group). The sham-operated rats which were used as the controlwere assigned into the normothermic control group (NC group) and moderate-hypothermic control group (HC group).The contents of glucose and ATP and activity of mitochondrial SDH were assayed at 0, 2, 6, 24, 48, 72 h after HIBD.Results The cerebal glucose contents in the IN and IH groups were significantly lower than those of the NC and Hcgroups 0 h after HIBD. Two hours after HIBD, the glucose content recovered to norhal level. The concentration of ATPand activity of SDH in the IN group decreased first, then they recovered gradually and reached a peak 72 hours afterHIBD. After 6 hours or 2 hours of HIBD, the ATP content or SDH activity of the brain in the IH group weresignificantly higher than those in the IN group. The ATP concentration was closely related to the SDH activity ( r =0.515, P ＜0.01). Conclusions Moderate hypothermia might protect brain tissue by inhibiting the decrease of SDHactivity and increasing the synthesis of ATP.
