Effect of Glycine on Serum IL-1 and IL-6 of NEC Rats Induced by Endotoxin and Hypoxia
10.3969/j.issn.1008-8830.2003.02.005
- VernacularTitle:甘氨酸对坏死性肠炎鼠血清IL-1和IL-6水平的影响
- Author:
LIU GUO-SHENG
1
;
YANG HUI
;
NIE CHUAN
;
LUO XIAN-QIONG
;
KANG JU-LING
;
GUAN JIE-BIN
;
LU DA-XIANG
Author Information
1. 暨南大学医学院附属第一医院(广州华侨医院)
- Keywords:
Glycine;
Endotoxin;
Hypoxia;
Necrotizing enterocolitis;
IL-1;
IL-6
- From:
Chinese Journal of Contemporary Pediatrics
2003;5(2):100-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of glycine on serum levels of IL-1 and IL-6 in rats with necrotizing enterocolitis (NEC) induced by endotoxin and hypoxia. Methods Forty SD rats were randomly assigned into the glycine-treated group and the normal saline (NS) control group. In the glycine-treated group, glycine (1 g/kg) was injected intravenously and lipopolysaccharide (LPS) of 2 mg/kg was administrated five minutes later. The control group rats were treated with the same volume of NS as a substitute for glycine. In both groups, 90 minutes after injection of LPS, FiO2 given was reduced from 21% to 5% and ventilation continued for 180 min or until the death of rats. At the end of the experiment, the blood samples and sections of the intestine were obtained immediately. Serum levels of IL-1 and IL-6 were measured using ELISA. The histopathological changes of the small intestine were studied. Results The survival time of the glycine-treated group was significantly longer than that of the control group [(159.25±22.78) min vs (138.75±19.05) min](P<0.01). The injury of the small intestine in the glycine-treated group was markedly alleviated (P<0.01). The levels of IL-1and IL-6 in the glycine-treated group were significantly lower than those in the control group [(149.1±76.1) ng/L vs (472.1±505.6) ng/L, (204.8±163.5) ng/L vs (585.8±574.5) ng/L, respectively] (P<0.01). Conclusions Glycine could reduce the levels of IL-1 and IL-6 and alleviate injuries of the intestine in rats with NEC induced by LPS and hypoxia.
