Differentiation-Inducing Effect of ATRA on Leukemia Cell Line K562
10.3969/j.issn.1008-8830.2003.01.003
- VernacularTitle:全反式维甲酸对K562细胞的诱导分化作用
- Author:
YUAN XIAO-JUN
1
;
LIAO QING-KUI
;
LUO CHUN-HUA
;
ZHAO YU-HONG
;
LI FENG-YI
;
ZHOU MI
;
YANG XIAN-JUN
Author Information
1. Second Affiliated Hospital West China University of Medical Sciences
- Keywords:
ATRA;
Leukemic cell;
K562;
Differentitation
- From:
Chinese Journal of Contemporary Pediatrics
2003;5(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective ATRA can restrain proliferation and promote differentiation in various tumor cells. The aim of the study is to investigate the differentiation characteristics of K562 induced by ATRA. Methods Morphology (Benzidine staining, Wright's staining, NSE staining and NBT recovery test) and flow cytometry were used to observe the differentiation characteristics of K562 after co-incubation with 1 μmol/L and 2.5 μmol/L ATRA for 1 d, 4 ds, and 5 ds. Results Co-incubated for 4 ds, 61.5% K562 cells in the 1 μmol/L ATRA group and 39% K562 cells in the 2.5 μmol/L ATRA group showed some evidence of myeloid maturation, but no evidence of erythroid or monocytoid maturation. Co-incubated for 5 ds, the percentage of differentiated K562 cells was much higher than that in the control group. One day after induction by 1 μmol/L ATRA or 2.5 μmol/L ATRA, the expression of CD13 was 8.0% and 6.7%, respectively, which was higher than that in the control group (2.1%). Five days after induction by 1 μmol/L ATRA or 2.5 μmol/L ATRA, the expression of CD13 increased to 28.1% and 37.8%, respectively, while the expression of CD71 decreased to 1.2% and 0.9% respectively. The differences between the ATRA groups and the control group were significant (P<0.05). CD71 decreased from 9.7% and 10.8% in the 1 μmol/L and 2.5 μmol/L ATRA groups on day 1 to 1.2% and 0.9% on day 5, while the CD13 expression level increased from 8.0% and 6.7% to 28.1% and 37.8%, respectively. Conclusions ATRA can induce K562 to differentiate into myeloid linage.
