Sequence Analysis and Genetic Variation of NS Gene of Human Parvovirus B19 Isolated from Chinese Children with Acute Aplastic Anemia
10.3969/j.issn.1008-8830.2003.01.002
- VernacularTitle:我国再障患儿血清中人细小病毒B19 NS基因序列测定及变异分析
- Author:
ZHANG GUO-CHENG
1
;
SU GUO-PING
;
SUN XIN
;
XU DONG-LIANG
;
CHEN CAI-PING
;
TANG XUE-QING
;
LI BIAO
Author Information
1. Xijing Hospital Forth Military Medical University
- Keywords:
Human parvovirus B19;
Gene;
Sequence analysis
- From:
Chinese Journal of Contemporary Pediatrics
2003;5(1):5-7,11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the NS gene structure and mutation of Chinese strains of human Parvovirus B19 (HPV B19). Methods NS genes of B19-XA17, XA18 and XA20 strains from three Chinese children with acute aplastic anemia were amplified by PCR, and then the amplified products were sequenced directly. Results Compared with the sequence of Au strain, four bases of NS genes of B19-XA17, XA18 and XA20 strains changed and their coding amino acids changed also. Conclusions Genetic diversity exists in mucleotide sequences of NS gene of HPV B19 isolated from the serum of Chinese children.
