Antiproliferative Effects of the Hemoregulatory Inhibitor N-acetyl-seryl-aspartyl-lysyl-proline on Rat Renal Interstitial Fibroblasts
10.3969/j.issn.1008-8830.2001.02.010
- VernacularTitle:造血细胞生长抑制因子抑制大鼠肾间质成纤维细胞的生长
- Author:
Hui-Jie XIAO
1
Author Information
1. First Hospital Beijing University
- From:
Chinese Journal of Contemporary Pediatrics
2001;3(2):158-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether the tetrapeptide N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP), a physiological hemorcgulatory inhibitor, has a biological function in non-hematopoietic cells (rat renal interstitial fibroblasts). Methods Cell proliferation was measured using standard cell culture techniques and the alama blue colorimctric assay. Results OD measurement of alamar blue absorption at 48 h was significantly inhibited in fibroblasts exposed to 1 μmol AcSDKP (P<0.01). During the 48-hour alamar blue absorption, only AcSDKP in concentrations of 10-7 mol/L, 10-6 mol/L, and 10-5 mol/L had significant inhibiting effects compared to cells grown in the 5% FBS RPMI 1640 media alone. The inhibition was dose-dependent, and disappeared at AcSDKP concentrations higher than 10-5 mol/L or lower than 10-8 mol/L. Maximum inhibition occurred at an AcSDKP concentration of 10-6 mol/L (33% suppression). Conclusions The inhibiting effect of AcSDKP is not limited to hematoposis cells. AcSDKP is specifically cleaved to an inactive form by the N-terminal active site of angiotensin converting enzyme (ACE) and ACE inhibitors (ACEI) can increase the concentration of AcSDKP. It suggests that AcSDKP may be an endogenous modulator of renal cell proliferation and the antiproliferative action of ACEI in renal diseases may be partly mediated by AcSDKP.
