Differentiation of Adult Rat Bone Marrow Stromal Cells Into NSE-positive Cells
10.3969/j.issn.1008-8830.2001.01.008
- VernacularTitle:成年大鼠骨髓基质细胞体外诱导分化为NSE阳性细胞
- Author:
Yan-Jie JIA
1
;
Yu-Jia YANG
;
Yuan-Zong SONG
Author Information
1. Xiangya Hospital Central South University
- From:
Chinese Journal of Contemporary Pediatrics
2001;3(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protocol that induces adult rat bone marrow stromal cells (MSCs) to express neuron-specific enolase (NSE) in vitro. Methods MSCs were preinduced with β-mercaptoethanol for 24 h, and then induced for 5 h. The positive percentages of NSE protein expression were measured by immunocytochemistry SABC staining. Results After the induction, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and processes which formed extensive networks. The positive percentage of NSE protein expression was (63.7±4.5)%. Conclusions β-mercaptoethanol may induce adult rat MSCs to NSE-positive cells in vitro.
