Ingredients of Panax notoginseng compound formula modulate inflam-mation and intestinal flora to attenuate endometrial fibrosis in rats with intrauterine adhesion
10.3969/j.issn.1000-4718.2024.11.018
- VernacularTitle:三七复方成分通过调节炎症反应和肠道菌群减轻宫腔粘连大鼠子宫内膜纤维化
- Author:
Mengchen TAN
1
;
Wen'e LIU
;
Lu MA
;
Yuxin ZENG
;
Xue'er YANG
;
Yaqi ZHAO
;
Ying PENG
;
Qiang AO
;
Hui LIU
;
Minyan LI
Author Information
1. 湖南中医药大学第一中医临床学院,湖南 长沙 410007
- Keywords:
intrauterine adhesion;
Panax notoginseng compound formula;
intestinal flora;
inflammation
- From:
Chinese Journal of Pathophysiology
2024;40(11):2135-2144
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of Panax notoginseng compound formula(PN)on endometrial fibro-sis by regulating inflammatory reaction and intestinal flora(IF)in a rat model of intrauterine adhesion(IUA).METHODS:The rat IUA model was established by following the mechanical injury method.A total of 50 rats were randomly divided in-to sham group,model group,low-dose(210 mg/kg)PN group,medium-dose(420 mg/kg)PN group and high-dose(840 mg/kg)PN group.After 8 weeks of intragastric administration,the uterus was collected to observe morphological changes with naked eye.The degree of uterine tissue damage and fibrosis was evaluated through hematoxylin-eosin(HE)and Mas-son staining.The collagen type Ⅰ(Col Ⅰ)was detected by immunohistochemistry.The interleukin-6(IL-6)and IL-10 pro-tein expression was detected by Western blot.The levels of IL-6,IL-1β,tumor necrosis factor-α(TNF-α)and vascular endothelial growth factor B(VEGFB)were detected by enzyme-linked immunosorbent assay(ELISA).The IF diversity and population structure were observed by 16S amplicon.RESULTS:Compared with the sham group,the uteruses of rats in the model group showed:reduced elasticity,accompanied by congestion and edema;decreased number of glands and blood vessels,and thinned endometrium(P<0.01);increased collagen fibers and Col Ⅰ protein expression(P<0.01);sig-nificantly increased IL-1β,IL-6,TNF-α and VEGFB levels in the uterine tissue(P<0.01);decreased IL-10 level(P<0.01);and reduced IF diversity(P<0.05).Compared with the model group,the drug intervention groups exhibited:re-covered elasticity of the uterus and relieved congestion and edema;increased number of endometrial glands and blood ves-sels(P<0.05);decreased collagen fibers and Col Ⅰ protein expression(P<0.01);reduced IL-1β,IL-6,and TNF-α lev-els to varying degrees in the uterine tissue(P<0.05);elevated IL-10 level(P<0.01);and improved IF diversity(P<0.05).CONCLUSION:The PN is able to significantly improve the endometrial tissue fibrosis in IUA rats.The under-lying mechanisms may be related to the inhibition of IL-6,IL-1β and TNF-α expression,up-regulation of IL-10,and im-provement of IF diversity.
