Analysis of interaction between estrogen receptor β and nuclear factor-κB in colorectal cancer
10.3969/j.issn.1000-4718.2024.11.007
- VernacularTitle:肠癌中雌激素受体β与核因子κB互作分析
- Author:
Yanjie PENG
1
;
Jinpei ZHANG
;
Jiaqi TIAN
;
Zhen CHEN
;
Liyang LIANG
;
Lin ZHANG
;
Dandan SONG
Author Information
1. 青岛大学附属山东省妇幼保健院妇女儿童疾病临床医学研究中心,山东省医药卫生出生缺陷防治与遗传医学重点实验室,山东 济南 250014
- Keywords:
colorectal cancer;
estrogen receptor β;
nuclear factor-κB;
protein-protein interaction
- From:
Chinese Journal of Pathophysiology
2024;40(11):2041-2049
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the interaction mechanisms of estrogen receptor β(ERβ),nuclear factor-κB(NF-κB)and activator protein-1(AP-1)in colorectal cancer by analyzing the transcriptome data after tumor necrosis fac-tor α(TNF-α)treatment and combining it with NF-κB/p65 and ERβ cistrome data in colon cancer cell lines HT29 and SW480.METHODS:The TNF-α transcriptome was integrated with p65 and ERβ cistrome data.Protein interaction net-works of TNF-α,NF-κB/p65 and ERβ were constructed in colon cancer cell lines HT29 and SW480 using R.RE-SULTS:TNF-α regulated genes through p65 DNA binding,which were mainly enriched in the NF-κB and mitogen-acti-vated protein kinase(MAPK)pathways.Components of the NF-κB/p65 and MAPK pathways had potential interactions with AP-1 family proteins.ERβ overexpression did not significantly affect TNF-α-mediated gene regulation but may regu-late AP-1 activity through the MAPK and phosphatidylinositol 3-kinase(PI3K)/Akt pathways.Furthermore,ERβ de-creased p65 DNA binding sites in HT29 but increased p65 binding sites in SW480,suggesting cell line-specific regulation of NF-κB by ERβ.CONCLUSION:In colorectal cancer,NF-κB,ERβ and AP-1 have potential interactions:TNF-α can regulate AP-1 through NF-κB,while ERβ overexpression can alter NF-κB-mediated regulation,and the influence of ERβ on NF-κB may be gender-related.
