lncRNA PCED1B-AS1 targets FUS to regulate MAPK signaling pathway and affects biological functions of papillary thyroid carcinoma cells
10.3969/j.issn.1000-4718.2024.11.005
- VernacularTitle:lncRNA PCED1B-AS1通过靶向FUS调控MAPK信号通路并影响甲状腺乳头状癌细胞生物学功能
- Author:
Jingjing XU
1
;
Fengyuan ZHANG
;
Jiazheng LI
;
Mi LI
;
Jiahui LIANG
;
Shengxia CHEN
Author Information
1. 江苏大学医学院检验医学系,江苏 镇江 212013
- Keywords:
papillary thyroid carcinoma;
long noncoding RNA PCED1B antisense strand 1;
fused in sarco-ma;
MAPK signaling pathway
- From:
Chinese Journal of Pathophysiology
2024;40(11):2022-2030
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects and mechanism of long noncoding RNA PCED1B antisense strand 1(lncRNA PCED1B-AS1)on the proliferation,migration,invasion and apoptosis of papillary thyroid carcinoma(PTC)cells.METHODS:Human PTC cells were cultured in vitro.The expression of PCED1B-AS1 and fused in sarcoma(FUS)was measured by RT-qPCR.The effects of knockdown/overexpression of PCED1B-AS1/FUS on the migration and invasion of PTC cells were detected via Transwell assay.The effects of knockdown/overexpression of PCED1B-AS1/FUS on PTC cell proliferation were analysed via CCK-8 and plate colony assay.The effect of knockdown PCED1B-AS1 on PTC cell apoptosis was determined by flow cytometry.The target binding of PCED1B-AS1 and FUS was determined with bioin-formatics and RNA immunoprecipitation(RIP)experiments.Fluorescence in situ hybridization experiment was performed to verify whether PCED1B-AS1 colocalises with FUS.The mitogen-activated protein kinase(MAPK)signaling pathway-re-lated proteins were detected via Western blot.RESULTS:(1)PCED1B-AS1 expression was significantly higher and FUS expression was significantly lower in PTC cells compared with normal thyroid Nthy-ori3-1 cell(P<0.05).(2)Knockdown of PCED1B-AS1 and overexpression of FUS inhibited PTC cell migration,invasion and proliferation,and promoted apopto-sis(P<0.05).(3)Bioinformatics analysis and RIP assay verified the existence of targeted binding of PCED1B-AS1 to FUS(P<0.05).(4)PCED1B-AS1 and FUS colocalised in the cytoplasm.(5)Inhibition of PCED1B-AS1 decreased the expression of MAPK signaling pathway-related proteins p-ERK 1/2,p-JNK and p-P38(P<0.05).CONCLUSION:ln-cRNA PCED1B-AS1 inhibits the proliferation,migration and invasion,and promotes the apoptosis of PTC cells,and its mechanism may be related to the expression of FUS and the MAPK signaling pathway.
