Celoside I enhances mitophagy of retinal ganglion cells in optic nerve in-jury model by regulating ROS-mediated JNK/c-Jun signaling pathway
10.3969/j.issn.1000-4718.2024.10.014
- VernacularTitle:青葙苷I通过调节ROS介导的JNK/c-Jun信号通路增强视神经损伤模型视网膜神经节细胞的线粒体自噬
- Author:
Yiyan HAN
1
,
2
;
Qu ZHENG
;
Lei ZHAO
;
Zhihao NING
;
Baoqiang DONG
;
Tao ZUO
Author Information
1. 辽宁中医药大学,辽宁 沈阳 110847
2. 辽宁省针灸养生康复重点实验室,辽宁 沈阳 110847
- Keywords:
celoside I;
optic nerve injury;
retinal ganglion cells;
mitophagy
- From:
Chinese Journal of Pathophysiology
2024;40(10):1898-1905
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aimed to investigate the mechanism by which celoside I enhances mitophagy in a model of optic nerve injury through regulation of reactive oxygen species(ROS)-mediated c-Jun N-terminal kinase(JNK)/c-Jun signaling pathway.METHODS:Twenty-four New Zealand white rabbits were randomly divided into four groups:sham surgery,model,mecobalamin,and experimental group.Optic nerve injury was induced in the model,mecobala-min,and experimental groups,while the sham surgery group underwent a sham procedure.The mecobalamin group re-ceived mecobalamin,the experimental group received celoside I,and the sham surgery and model groups received saline.Interventions were administered daily for 28 d.Various techniques including endoscopy,hematoxylin-eosin(HE)stain-ing,TUNEL method,immunofluorescence staining and Western blot were used to assess fundus condition,retinal mor-phology,apoptosis,ROS expression,and protein levels in the retina.RESULTS:Fundus examination revealed im-proved blood flow in the mecobalamin and experimental groups compared to the model group.Retinal morphology showed enhanced retinal ganglion cells(RGCs)in the mecobalamin and experimental groups.Apoptosis index was lower in the mecobalamin group compared to the experimental group.Immunofluorescence staining indicated reduced ROS and P62 ex-pression and increased parkin and microtubule-associated protein light chain 3(LC3)expression in the experimental group compared to the mecobalamin group.Protein analysis showed decreased JNK,c-Jun,and P62 levels,and increased parkin and LC3 levels in the mecobalamin and experimental groups compared to the model group.CONCLUSION:Celo-side I reduces ROS expression,inhibits the JNK/c-Jun pathway,enhances mitophagy,reduces apoptosis,and protects RGCs in optic nerve injury models.
