Mechanism of Sanguis draconis flavones in treatment of myocardial ischemia-reperfusion injury based on network pharmacology
10.3969/j.issn.1000-4718.2024.10.010
- VernacularTitle:基于网络药理学研究龙血竭总黄酮治疗心肌缺血再灌注损伤的作用机制
- Author:
Sheng LI
1
,
2
,
3
;
Liudan LIANG
;
Yan LIU
;
Gencheng LIANG
;
Wenlin LUO
;
Zhaohe HUANG
Author Information
1. 右江民族医学院附属医院,广西 百色 533000
2. 右江民族医学院研究生学院,广西 百色 533000
3. 右江民族医学院附属医院动脉粥样硬化和缺血性心血管疾病实验室,广西 百色 533000
- Keywords:
Sanguis draconis flavones;
myocardial ischemia reperfusion injury;
network pharmacology;
Lou-reirin B;
ALOX15
- From:
Chinese Journal of Pathophysiology
2024;40(10):1864-1873
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To predict the mechanism of Sanguis draconis flavones(SDF)in the prevention and treat-ment of myocardial ischemia reperfusion injury(MIRI)based on network pharmacology and molecular docking methods.METHODS:The main chemical constituents of SDF were collected through literature search,and the targets of key con-stituents were screened by using the SwissTargetPrediction and TargetNet databases.Disease targets were also screened based on GeneCards,OMIM,TTD and PharmGkb databases,then targets were intersected with Cytoscape to construct the"drug-key constituent-target"network diagram,and the core target was obtained through visualization and analysis by Cytoscape software.Gene Ontology(GO)functional enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were analyzed by the Metascape platform.By utilizing AutoDock Vina software and Pymol,molecular docking between core compounds and core targets was carried out.Further,animal experiments were performed to explore the pharmacodynamic mechanism of SDF.RESULTS:The active constituents of SDF included loureirin B and loureirin A,which were mapped to 391 targets.A total of 3 096 MIRI disease targets were obtained from the database,af-ter intersection,172 intersection targets were obtained,and 56 core targets were acquired through analysis.The core relat-ed pathways included the cancer pathway and cell death signaling pathway.The results of molecular docking verified the strong binding activity between key constituents and key targets.Animal experiments demonstrated that SDF effectively prevented and treated MIRI,significantly inhibited the arachidonic acid 15-lipoxygenase(ALOX15)mRNA and protein expression,and reduced the myocardial infarction size after MIRI.CONCLUSION:SDF may play a positive role in the treatment of MIRI,which may be related to the regulation of the ALOX15 factor.
