Huangqi-Danggui decoction alleviates rat cerebral ischemia-reperfusion in-jury by regulating macroautophagy and chaperone-mediated autophagy
10.3969/j.issn.1000-4718.2024.08.010
- VernacularTitle:黄芪-当归通过调控巨自噬和分子伴侣介导的自噬缓解大鼠脑缺血/再灌注损伤
- Author:
Luyao LIU
1
;
Yi ZHANG
;
Yihang LI
;
Yijie LIU
;
Yuxin GE
;
Hongfei DU
;
Wen YUAN
;
Weijuan GAO
Author Information
1. 河北中医药大学河北省心脑血管病中医药防治研究重点实验室,石家庄 河北 050091
- Keywords:
Huangqi-Danggui decoction;
cerebral ischemia/reperfusion injury;
macroautophagy;
chaperone-mediated autophagy
- From:
Chinese Journal of Pathophysiology
2024;40(8):1436-1445
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Huangqi-Danggui decoction(HQDG)on the brain tissue of rats with cerebral ischemia/reperfusion(I/R)injury for 7 d by regulating macroautophagy and chaperone-mediated autophagy(CMA),and to explore its mechanism.METHODS:Male SD rats were randomly divided into sham group,model group,HQDG group and Xuesaitong(XST)group.Determination of main chemical components of HQDG by liquid chro-matography-mass spectrometry.The model of middle cerebral artery occlusion/reperfusion in rats was established by the left modified thread embolism method,and the changes of cerebral blood flow were observed by laser speckle blood flow imager.Zea Longa score was used to observe the neurological deficit.HE staining was used to observe the degree of nerve cell injury.The changes of neurovascular unit and autophagosomes in brain tissue were observed by transmission electron microscopy.Immunohistochemical method was used to detect the expression of LC3,P62,lysosome-associated membrane protein-2A(LAMP-2A),heat shock protein 70(HSP70)and myocyte enhancer factor 2D(MEF2D)proteins.Western blot was used to detect the expression of autophagy-related proteins P62 and LC3-Ⅱ/LC3-I.RESULTS:Compared with the sham group,the neurological deficit score in model group was significantly higher(P<0.01).A large number of nerve cells showed necrosis and nuclear dissolution,with the cell arrangement being disordered.The number of autophagosomes increased.The protein expression levels of LC3,LAMP-2A,HSP70 and MEF2D in brain tissue increased,while the ex-pression level of P62 protein decreased(P<0.05 or P<0.01).Compared with the model group,the scores of neurological deficit in brain tissue in HQDG and XST groups were significantly lower(P<0.01).Cell damage was significantly re-duced.The number of autophagosomes further increased.The expression levels of LAMP-2A,HSP70,MEF2D and P62 proteins in brain tissue decreased,while the expression levels of LC3-Ⅱ/LC3-I protein increased(P<0.05 or P<0.01).CONCLUSION:HQDG can alleviate cerebral ischemia/reperfusion injury in rats and exert neuroprotective effects by ac-tivating macroautophagy and reducing CMA.
