Effect of heptamethoxyflavone on proliferation,migration and invasion of human colorectal cancer cells and its mechanism
10.3969/j.issn.1000-4718.2024.08.005
- VernacularTitle:七甲氧基黄酮抑制人结直肠癌细胞增殖、迁移和侵袭的机制研究
- Author:
Shiqi XU
1
;
Yingtong CHEN
;
Man ZHUANG
;
Gengxin YU
;
Xiaoyan WANG
;
Yi CAI
;
Shaoju GU
Author Information
1. 广州医科大学公共卫生学院,广东 广州 511436
- Keywords:
heptamethoxyflavone;
colorectal cancer;
cell proliferation;
cell migration;
cell invasion;
oxida-tive stress
- From:
Chinese Journal of Pathophysiology
2024;40(8):1392-1398
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study is aimed to investigate the impact of 3,5,6,7,8,3',4'-heptamethoxyflavone(HMF)on the proliferation,invasion,and migration of human colorectal cancer(CRC)cell lines(SW480 and HCT116)and preliminarily explore the underlying molecular mechanisms.METHODS:Human colorectal cancer cells(SW480 and HCT116)cultured in vitro were subjected to various concentrations of HMF(0,12.5,25 and 50 μmol/L)for 48 h.Proliferation levels were assessed using the CCK-8 assay,invasion abilities were examined via the Transwell assay,migra-tion rates were measured using the scratch assay,and oxidative stress levels were determined by the DCF-DA reactive oxy-genation assay.The mRNA expression levels of heme oxygenase-1(HO-1)mRNA and NAD(P)H:quinone oxidoreduc-tase-1(NQO-1)were quantified using RT-qPCR.RESULTS:Treatment with varying concentrations of HMF resulted in a significant reduction in the proliferative capacity of SW480 and HCT116 cancer cells,as was indicated by CCK-8 experi-ments(P<0.05).Transwell assays demonstrated a pronounced attenuation in the invasive potential of SW480 and HCT116 following HMF treatment(P<0.05).Scratch assays highlighted a notable constraint on the migratory capabilities of SW480 and HCT116 after HMF treatment(P<0.05).DCF-DA staining revealed a substantial increase in reactive oxy-gen species(ROS)levels within SW480 and HCT116 cells after HMF treatment(P<0.05).Furthermore,RT-qPCR ex-periments elucidated that HMF markedly suppressed the mRNA expression of antioxidant genes HO-1 and NQO-1.CON-CLUSION:HMF induces oxidative stress response in SW480 and HCT116 cells,consequently inhibiting their prolifera-tion,invasion and migration.
