High expression of vitamin D receptor in lung adenocarcinoma and its in-fluence on viability and metastasis ability of lung adenocarcinoma cells
10.3969/j.issn.1000-4718.2024.08.004
- VernacularTitle:维生素D受体在肺腺癌高表达及其对肺腺癌细胞活力和转移能力影响的研究
- Author:
Shumei ZOU
1
;
Zhenming BAO
;
Jia YE
;
Zongyang YU
;
Yingfang SONG
Author Information
1. 联勤保障部队第900医院呼吸内科,福建 福州 350025
- Keywords:
vitamin D receptor;
lung adenocarcinoma;
cell viability;
cell invasion;
cell migration
- From:
Chinese Journal of Pathophysiology
2024;40(8):1384-1391
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the expression level of vitamin D receptor(VDR)in lung adenocarcinoma and its impact on the biological function of lung adenocarcinoma cells.METHODS:The mRNA expression level of VDR in lung adenocarcinoma and its relationship with prognosis were analyzed through 6 lung adenocarcinoma datasets(compris-ing a total of 792 lung adenocarcinoma tissues and 230 adjacent non-tumor tissues).Immunohistochemistry was used to de-tect VDR protein expression in 30 lung cancer patients.Lung adenocarcinoma cell lines A549 and H1650 were studied,with transfection of negative control(NC)or two VDR short hairpin RNAs(VDR-shRNAs).CCK-8 assay compared the cell viability of cells in each experimental group.Transwell and wound-healing assays compared the invasion and migra-tion capabilities.Gene set enrichment analysis identified pathways enriched in lung adenocarcinoma tissues with high VDR expression.RESULTS:The mRNA expression level of VDR was significantly increased in lung adenocarcinoma tissues(P<0.01).Immunohistochemistry further confirmed the high expression of VDR in lung adenocarcinoma(P<0.01).The survival analysis showed that the expression of VDR had no significant effect on the overall survival of lung ad-enocarcinoma patients(P>0.05).Knockdown of VDR significantly inhibited the cell viability,invasion,and migration ca-pacity of lung adenocarcinoma cells(P<0.05).Gene set enrichment analysis showed that lung adenocarcinoma tissues with high VDR expression were enriched in signaling pathways such as epithelial-mesenchymal transition(P<0.05).CONCLUSION:VDR is highly expressed in lung adenocarcinoma,and VDR knockdown can inhibit the cell viability,in-vasion,and migration capacity of lung adenocarcinoma cells.
