miRNA-126 reverses cisplatin resistance in lung cancer A549/DDP cells by inhibiting autophagy
10.3969/j.issn.1000-4718.2024.08.003
- VernacularTitle:miRNA-126通过抑制自噬逆转肺癌A549/DDP细胞顺铂耐药的作用研究
- Author:
Xiali TANG
1
;
Jun CHEN
;
Demin JIAO
;
Xiang LIU
Author Information
1. 中国人民解放军联勤保障部队第九〇三医院,浙江 杭州 310004
- Keywords:
microRNA-126;
lung cancer;
autophagy;
cisplatin;
drug resistance
- From:
Chinese Journal of Pathophysiology
2024;40(8):1378-1383
- CountryChina
- Language:Chinese
-
Abstract:
AIM:Autophagy levels in lung cancer cisplatin-resistant A549/DDP cells and cisplatin-sensitive A549 cells were compared.The effects of microRNA-126(miRNA-126)on autophagy in lung cancer cisplatin-resistant cells were assessed,and the role of miRNA-126 in lung cancer cisplatin-resistant cells was discussed.METHODS:An MTT assay was used to detect the half maximal inhibitory concentration of cisplatin.The miRNA-126 expression was de-tected by RT-qPCR.Autophagolysosomes were observed under a fluorescence microscope using acridine orange staining.Protein levels of autophagy-associated molecule microtubule-associated protein 1 light chain 3(LC3)in lung cancer cells were determined by Western blot.RESULTS:Autophagy was significantly elevated in A549/DDP cells compared with A549 cells(P<0.01).The expression level of miRNA-126 was significantly elevated in A549/DDP cells after transfection with miRNA-126(P<0.01).miRNA-126 reversed cisplatin resistance of A549/DDP cells,and the level of intracellular acid dye follicular bright red fluorescence in the A549/DDP cells was significantly reduced after transfection with miRNA-126(P<0.01).The LC3-Ⅱ protein level in A549/DDP cells was significantly reduced after transfection with miRNA-126(P<0.01),and inhibition of cell autophagy by 3-methyladenine(3-MA)enhanced cisplatin sensitivity of A549/DDP cells(P<0.01).Compared with 3-MA+NC group,miRNA-126 combined with 3-MA further increased cisplatin sensitivity of A549/DDP cells(P<0.01).CONCLUSION:miRNA-126 reverses the cisplatin resistance in lung cancer A549/DDP cells by inhibiting autophagy.
