Differential expression of ultraconserved RNA uc.102-uc.106 cluster and its host gene OLA1 in various tissues
10.3969/j.issn.1000-4718.2024.08.001
- VernacularTitle:超保守RNA uc.102-uc.106及其宿主基因OLA1在不同组织中的差异表达
- Author:
Xin WANG
1
;
Yi ZHANG
;
Han XU
;
Zichen WEI
;
Lei PANG
;
Mingchao ZHAO
;
Duonan YU
Author Information
1. 扬州大学医学院/江苏省非编码RNA基础与临床转化重点实验室,江苏 扬州 225009
- Keywords:
ultraconserved RNA;
uc.102-uc.106 gene cluster;
OLA1 gene;
C57BL/6J mice
- From:
Chinese Journal of Pathophysiology
2024;40(8):1361-1368
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate the expression differences of the ultraconserved RNA gene cluster uc.102-uc.106 and its host gene Obg-like ATPase 1(OLA1)in various tissues of C57BL/6J mice.METHODS:Five healthy male C57BL/6J mice aged 8～10 weeks were selected,and under normal physiological conditions,various tis-sues and organs,including liver,testis,bone marrow,brain,kidney,blood,lung,colon,thymus,spleen,stomach,heart,lymph nodes and bladder,were collected.Simultaneously,magnetic bead separation technology was employed to extract bone marrow blood cells,including B cells,macrophages,erythroid precursor,and mature red blood cells.The ex-pression levels of uc.102-uc.106 and OLA1 mRNA were determined by RT-qPCR with GAPDH as the internal reference.RESULTS:The OLA1 mRNA exhibited high expression levels in the brain,lymph nodes,testis and thymus of C57BL/6J mice,and low expression levels in the liver,spleen,lung,colon and peripheral blood(P<0.05).The expression trend of the uc.102-uc.106 gene cluster was generally opposite to that of OLA1 mRNA,except for both exhibiting high expression levels in testicular tissue(P<0.05).In blood cells,the OLA1 mRNA and the uc.102-uc.106 gene cluster showed the highest expression levels in B cells and the lowest expression levels in mature red blood cells(P<0.05).CONCLU-SION:This study indicates that OLA1 gene and uc.102-uc.106 gene cluster are expressed in different tissues and blood cells of C57BL/6J mice.Notably,the high expression levels of OLA1 gene and uc.102-uc.106 gene cluster in the mouse testis suggest a potential association with male reproductive functions.
