Compound ANBP promotes diabetic wound healing by regulating TCA circulation-related enzymes
10.3969/j.issn.1000-4718.2024.07.017
- VernacularTitle:复方ANBP通过调控TCA循环相关酶促进糖尿病创面愈合
- Author:
Wei WANG
1
;
Qian HOU
;
Xinling HUANG
;
Jun WANG
;
Meilin ZOU
;
Yarong DING
;
Letian GUO
;
Chenlei XIE
;
Xinyu HUANG
;
Li CHEN
;
Zhongzhi ZHOU
Author Information
1. 湖南中医药大学中西医结合学院,湖南 长沙 410208
- Keywords:
diabetes mellitus;
wound healing;
tricarboxylic acid cycle;
mitochondria
- From:
Chinese Journal of Pathophysiology
2024;40(7):1283-1291
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of the compound ANBP on wound healing in diabetic rats and ex-plore its mechanism of action.METHODS:Ninety male SD rats were randomly divided into blank,model,compound ANBP,Beifuxin,and nicotinamide mononucleotide(NMN)groups,with 16 rats in each group.Wound healing in each group was observed and samples were taken on days 3,7 and 14 to analyze the wound healing rate.Local histopathological changes were observed using HE and Masson staining.The expressions of pyruvate dehydrogenase E1 subunit alpha 1(PDHA1),citrate synthase(CS),isocitrate dehydrogenase(IDH1)and oxoglutarate dehydrogenase(OGDH)were de-tected through immunofluorescence and Western blot.The number and morphology of mitochondria in the wound tissue were observed using transmission electron microscopy.RESULTS:Histomorphological changes revealed significant im-provement in diabetic wound healing in the blank and compound ANBP groups compared to that of the model group.The wound healing rates of the blank,compound ANBP,Beifuxin,and NMN groups were significantly increased on days 3,7,and 14(P<0.01).Compared to the model group,granulation tissue generation was higher in the other groups,cover-ing the wound defect and producing abundant collagen fibers.At 3,7,and 14 days after intervention,the blank,com-pound ANBP,Beifuxin,and NMN groups showed significantly enhanced fluorescence intensities of TCA cycling-related enzymes PDHA1,CS,IDH1,and OGDH indicating increased expression of these enzymes.The levels of the TCA cy-cling-related enzymes were significantly increased(P<0.01)in the compound ANBP,Beifuxin and NMN groups but were significantly decreased(P<0.01)in the model group.An increase in the number and density of mitochondria and a de-crease in the cavitation rate of mitochondria with improved morphology(P<0.05)was observed in the group treated with compound ANBP.CONCLUSION:Compound ANBP may increase the number of mitochondria,improve mitochondrial morphology and function,upregulate the expression levels of PDHA1,CS,IDH1,and OGDH proteins,and accelerate the regeneration of wound granulation tissue,thus promoting the healing of diabetic wounds in rats.
