Exploring optimal tamoxifen labeling dose in myocardial cell prolifera-tion using Brainbow tracing mice
10.3969/j.issn.1000-4718.2024.06.023
- VernacularTitle:Brainbow示踪小鼠心肌细胞增殖中他莫昔芬最适标记剂量的探究
- Author:
Yangyang GAO
1
;
Jia GAO
;
Meng SUN
;
Rui WANG
Author Information
1. 山西医科大学第一临床医学院,山西 太原 0300001
- Keywords:
Brainbow mice;
myocardial cells;
cell proliferation;
tamoxifen;
fluorescent labeling
- From:
Chinese Journal of Pathophysiology
2024;40(6):1147-1152
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study employs Brainbow 2.1 mice to trace myocardial cell lineage and investigates the optimal dose of tamoxifen for labeling proliferating myocardial cells.METHODS:Four-week-old Brainbowfl/+;Myh6-Mer-CreMer+mice were administered tamoxifen at three different doses(3,9 and 27 mg/kg)via intraperitoneal injection to in-duce Cre recombinase expression and label myocardial cells with specific colors.Four weeks later,the samples were stained,and the number of myocardial cells labeled with varying doses of tamoxifen was quantified under confocal micro-scope.This quantification included the proportion of individually labeled cells,adjacent clusters of two cells,and clusters of three or more cells.An appropriate dose(9 mg/kg)of tamoxifen for labeling Brainbow mice was selected to compare myocardial cell proliferation between sham and myocardial infarction(MI)model groups.RESULTS:(1)After 9 mg/kg tamoxifen injection,the proportion of individually labeled cells was 99.13%±0.03%,that of adjacent 2-cell clusters was 0.82%±0.09%,and that of≥3-cell clusters was 0.05%±0.01%.The 3 mg/kg group had fewer total labeled cells,while the 27 mg/kg group showed significant clonal expansion and enrichment.(2)After labeling myocardial cells with 9 mg/kg tamoxifen,the MI mice showed increased proportions of 2-cell and≥3-cell clusters compared with sham group(P<0.01),indicating an increase in myocardial cell proliferation after MI.CONCLUSION:The proportion of adjacent myocardial cell clusters labeled with 9 mg/kg tamoxifen more closely approximates the physiological myocardial cell proliferation rate,indicating 9 mg/kg as the most suitable tamoxifen dose for tracing myocardial cell proliferation.
