Effects of sodium hydrosulfide on HK2-NLRP3-GSDMD pathway and pyroptosis induced by lung ischemia/reperfusion in rats
10.3969/j.issn.1000-4718.2024.06.017
- VernacularTitle:硫氢化钠对大鼠肺缺血/再灌注引发的细胞焦亡及HK2-NLRP3-GSDMD通路的影响
- Author:
Lu SHI
1
;
Xiaoting WANG
;
Zhenzhen LUO
;
Jun CHENG
;
Sian CHEN
;
Jun-Peng XU
;
Qihao ZHANG
;
Wenjie CAO
;
Man HUANG
;
Yunna TIAN
;
Xuguang JIA
;
Wantie WANG
Author Information
1. 宜宾学院体育与大健康学院,四川 宜宾 644000
- Keywords:
lung ischemia/reperfusion injury;
sodium hydrosulfide;
glycolysis;
pyroptosis;
HK2-NLRP3-GSDMD pathway
- From:
Chinese Journal of Pathophysiology
2024;40(6):1105-1113
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of sodium hydrosulfide(NaHS)on hexokinase 2(HK2)-nucleo-tide-binding oligomerization domain-like receptor protein 3(NLRP3)-gasdermin D(GSDMD)pathway and pyroptosis in-duced by lung ischemia/reperfusion(I/R)in rats.METHODS:Male Sprague-Dawley rats were divided into 6 groups:control group,control+NaHS group,I/R group,low-dose NaHS+I/R(L+I/R)group,medium-dose NaHS+I/R(M+I/R)group,and high-dose NaHS+I/R(H+I/R)group,with 6 rats in each group.The NaHS was administered via intraperi-toneal injection at 1.5 mL,30 min before modeling.The left lung tissues were collected 30 min after ischemia and 1 h af-ter reperfusion,and the wet/dry weight ratio and total lung water content were recorded.Hematoxylin-eosin(HE)staining was used to examine lung tissue morphological changes.The levels of malondialdehyde(MDA),myeloperoxidase(MPO)and lactate in lung tissues were measured with test kits.ELISA was employed to determine the levels of interleukin-1β(IL-1β)and IL-18.The expression of glycolysis-and pyroptosis-related indicators was analyzed by Western blot,qRT-PCR and immunofluorescence staining.RESULTS:Compared with control group,the rats in NaHS group showed no signifi-cant differences in all laboratory tests(P>0.05).The rats in I/R group exhibited significant lung injury,oxidative stress,increased lactate level,and up-regulated glycolysis and pyroptosis(P<0.05 or P<0.01).Compared with I/R group,the indicators in L+I/R group showed a downward trend(P<0.01)or no difference(P>0.05),while those in M+I/R group dis-played a significant reduction(P<0.05 or P<0.01).However,the indexes in H+I/R group exhibited no significant dif-ferences in these tests(all P>0.05).CONCLUSION:A moderate dose(56 μmol·L-1·kg-1)of NaHS mitigated the oc-currence of pyroptosis by inhibiting the HK2-NLRP3-GSDMD pathway,thus contributing to the attenuation of lung I/R in-jury in rats.
