Interference with Gal-1 inhibits EMT and migration of human breast cancer MDA-MB-231 cells via TGF-β pathway
10.3969/j.issn.1000-4718.2024.06.014
- VernacularTitle:干扰Gal-1通过TGF-β通路抑制人乳腺癌MDA-MB-231细胞的EMT和迁移
- Author:
Shizhong REN
1
;
Xuyong QIN
;
Guoli ZHOU
;
Wei ZHAO
;
Shujun CAO
;
Zhenyu MIAO
;
Chengping LI
Author Information
1. 聊城大学生命科学学院,山东 聊城 252000
- Keywords:
Galectin-1;
epithelial-mesenchymal transition;
transforming growth factor-β;
MDA-MB-231 cells;
cell migration;
cell proliferation
- From:
Chinese Journal of Pathophysiology
2024;40(6):1078-1084
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect and mechanism of the interfering Gal-1 on epithelial-mesenchymal transition(EMT),migration and proliferation in MDA-MB-231 cells via transforming growth factor-β(TGF-β)pathway.METHODS:The stable cell lines(shGal-1)which Gal-1 expression were inhibited completely and their control cell lines were used as experimental cells.Western blot assay was used to detect the effects of shGal-1 on EMT process of MDA-MB-231 cells after TGF-β treatment;The effect of shGal-1 on cell migration and invasion after TGF-β treatment was verified by cell scratch and transwell test;The effect of shGal-1 on the TGF-β pathway related proteins were detected by western blot;Finally,the effect of shGal-1 on cell proliferation was detected by MTT and western blot.RESULTS:shGal-1 inhib-ited TGF-β-mediated EMT in MDA-MB-231 cells and regulated phosphorylation of pathway signaling molecules(ERK,AKT and GSK3β);shGal-1 could inhibit the proliferation of MDA-MB-231 cells.CONCLUSION:shGal-1 can inhibit the TGF-β-mediated EMT,migration and proliferation of MDA-MB-231 cells.
