Peiminine inhibits viability of human colonic adenocarcinoma SW480 cells by down-regulating expression of CDK2/CDK4/CDK6 and cyclin D1
10.3969/j.issn.1000-4718.2024.06.013
- VernacularTitle:贝母素乙通过下调CDK2/CDK4/CDK6和cyclin D1表达抑制人结肠腺癌SW480细胞活力
- Author:
Xia YANG
1
;
Yaru LI
;
Yue LI
;
Hongyue MAO
;
Bing BAI
;
Yiquan LI
;
Ji-Cheng HAN
;
Yining WAN
;
Shimin XIE
;
Yilong ZHU
;
Ningyi JIN
Author Information
1. 延边大学医学院病理学与病理生理学教研室,吉林 延吉 136200
- Keywords:
SW480 cells;
peiminine;
cyclin-dependent kinases;
cell cycle
- From:
Chinese Journal of Pathophysiology
2024;40(6):1070-1077
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study examined the inhibitory effect of peiminine on the human colonic adenocarcino-ma cell line SW480 and explored the underlying mechanisms.METHODS:SW480 and human normal colonic epithelial CCD-841CoN cells were treated with different concentrations of peiminine and subjected to the CCK-8 assay to select the optimal treatment time and concentration of the compound.SW480 cell migration and invasion were evaluated by the wound-healing and Transwell assays.Cell cycle progression was analyzed by flow cytometry.The expression levels of cell cycle-related proteins were examined by Western blot.SW480 xenograft tumor model was established in nude mice to ex-amine the effect of peiminine on tumor growth and the expression of cell cycle-related proteins in vivo.RESULTS:Peimi-nine(110 mg/L)significantly inhibited the proliferation of SW480 cells compared with the control group(P<0.01),caused cell cycle arrest at G1 phase,and significantly downregulated the expression of cyclin dependent kinase 2(CDK2),CDK4,CDK6,cyclin D1,p-Rb/Rb,E2F1,E2F3,and E2F4(P<0.05).Peiminine inhibited SW480 xenograft tumor growth,prolonged the survival of model mice,and affected the expression of CDK2,CDK4,CDK6,and cyclin D1 in tu-mor tissues.CONCLUSION:Peiminine promotes G1 phase arrest by down-regulating the expression of CDK2,CDK4,CDK6,and cyclin D1,thereby inhibiting the proliferation of SW480 cells.
