Preliminary serum pharmacological exploration of method to preparate Ganlong capsule-containing serum
10.3969/j.issn.1000-4718.2024.05.023
- VernacularTitle:基于血清药理学的肝龙胶囊含药血清制备方法的初步探究
- Author:
Keke ZHANG
1
;
Hao BAI
;
Jinyu LÜ
;
Yue JIN
;
Rui LUO
Author Information
1. 暨南大学基础医学与公共卫生学院病理生理学系,国家中医药管理局病理生理实验室,广东 广州 510632
- Keywords:
Ganlong capsule;
drug-containing serum;
blood collection time;
volume fraction
- From:
Chinese Journal of Pathophysiology
2024;40(5):956-960
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the optimal time of blood collection and the best concentration of Ganlong capsule(GLC)-containing serum for steatohepatitis.METHODS:Sixty C57BL/6J mice were gavaged with GLC at a dose of 90 mg/kg body weight,twice a day for 7 days.Control mice were administered the same volume of 0.9%saline.After 0.5,1,2 and 4 h from the last gavage,blood was collected to prepare GLC-containing serum.The LC-MS/MS was used to detect the inosine content of blank and drug-containing serum at different blood collection times to determine the optimal time.Subsequently,NCTC1469 cells stimulated with palmitic acid(PA)were treated with different concentrations of the GLC-containing serum.Nile Red staining was used to detect lipid droplet formation,and RT-qPCR was employed to as-sess the expression of intracellular fatty acid synthesis(Fabp1,Scd1)and fatty acid β-oxidation gene(Pparα).RE-SULTS:(1)LC-MS/MS detection revealed that the inosine concentration peaked 2 h after the last administration.(2)Nile red staining demonstrated that the GLC-containing serum exerted a dose-dependent decreasing trend of lipid droplets in hepatocytes induced by PA.(3)GLC-containing serum significantly downregulated the mRNA expression levels of lipo-genesis-genes(Fabp1 and Scd1)with a dose-dependent decreasing trend in the PA-induced NCTC1469 cells.The mRNA expression of genes associated with fatty acid β-oxidation(Pparα)were upregulated with a dose-dependent increasing trend.CONCLUSION:The 10%GLC-containing serum collected 2 hours after the last administration in mice has the best therapeutic effect for steatohepatitis.
