Improvement Effect of Danggui ShaoYao San on the Reproductive Dysfunction Model in Drosophila Melanogaster
10.3870/j.issn.1004-0781.2024.12.007
- VernacularTitle:当归芍药散对果蝇生殖功能损伤模型的改善作用
- Author:
Yongqing HUA
1
,
2
,
3
;
Yuqi MEI
;
Xinyue ZHAO
;
Shaoqin MO
Author Information
1. 江苏省中药资源产业化过程协同创新中心,南京 210023
2. 南京中医药大学药学院,南京 210023
3. 江苏省中药药效与安全性评价重点实验室,南京 210023
- Keywords:
Danggui Shaoyao San;
Tripterygium glycosides;
Reproductive function injury
- From:
Herald of Medicine
2024;43(12):1913-1918
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a drosophila reproductive dysfunction model and observe whether Danggui Shaoyao San(DSS)has an improving effect.Methods Tripterygium glycosides were used to establish the drosophila reproductive dysfunction model.The number of offspring pupae,the parental superoxide dismutase(SOD)and catalase(CAT)activities,the parental reproductive-related gene expression,the F1 generation body weight,and the F1 generation development-related genes were measured to evaluate the efficacy of DSS.Results Tripterygium glycosides in a dose of 20 mg·mL-1 significantly inhibited the quantity of offspring(P<0.01)and were suitable for establishing the drosophila reproductive dysfunction model.Compared with the model control group,medium(10 mg·mL-1)and high(20 mg·mL-1)dose DSS significantly increased the number of offspring pupae(P<0.01,P<0.05).High-dose DSS significantly increased the SOD and CAT activities of the parent drosophila(P<0.01).Medium and high doses of DSS promoted the expression of drosophila parental reproduction-related genes,among which the medium dose DSS increased the mRNA expression of DEAD-Box helicase 4(VASA)and factor in the germline alpha(Figla)(P<0.05,P<0.01),and the high dose DSS increased the mRNA expression of VASA,Figla and forkhead box O(FoxO)(P<0.01).The medium dose of DSS significantly increased the body weight of F1-generation female drosophila(P<0.05),and the high dose of DSS significantly increased the body weight of both F1-generation female and male drosophila(P<0.05,P<0.01).Both the medium and high dose DSS increased the mRNA expression of the F1 generation development-related gene mammalian target protein of rapamycin(mTOR)(P<0.01).Conclusions DSS improves the reproductive function injury of drosophila induced by tripterygium glycosides.
