Proliferative Effect of Youguiyin and its Main Constituents Absorbed in the Blood on Rats Osteoblasts
10.3870/j.issn.1004-0781.2024.12.003
- VernacularTitle:右归饮及其入血成分对大鼠成骨细胞的促增殖作用
- Author:
Yaoyao ZHU
1
;
Xi ZHU
;
Jianhua ZHANG
;
Hua YIN
Author Information
1. 浙江中医药大学药学院中药标准化研究实验室,杭州 311400
- Keywords:
Youguiyin;
Constituents absorbed in the blood;
Osteoblasts;
Proliferation
- From:
Herald of Medicine
2024;43(12):1881-1887
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the main blood absorbed constituents from Youguiyin and elucidate the proliferative effect of Youguiyin and its main blood absorbed constituents on osteoblasts in rats.Methods Ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)technique was used to establish the analysis method for Youguiyin,individual herbal medicines and the medicated serum containing Youguiyin after oral administration in Sprague-Dawley rats.Osteoblasts of rats were isolated and cultured in vitro.The effect of Youguiyin,medicated serum,and its main blood absorbed components on osteoblast proliferation rate was evaluated by thiazole blue(MTT)assay.Results Ten components were detected in the blood serum of rats after oral administration of Youguiyin,namely aconitine,hypaconitine,mesaconitine,benzoylaconitine,benzoylhypaconitine,benzoylmesaconine from Aconiti Lateralis Radix Praeparata,cinnamic acid from Cinnamomi Cortex,morroniside,loganin from Corni Fructus,and pinoresinoldiglucoside from Eucommiae Cortex,respectively.Youguiyin decoction and seven components from Aconiti Lateralis Radix Praeparata and Cinnamomi Cortex showed a certain promoting proliferation effect on osteoblasts when the concentrations were 1 μg·mL-1,10 ng·mL-1,10 ng·mL-1,1 ng·mL-1,100 μg·mL-1,100 pg·mL-1,1 μg·mL-1 and 1 ng·mL-1,respectively.Besides,the effect onset was relatively rapid.The onset time of medicated serum containing Youguiyin was slow.Conclusions The ten chemical components absorbed in the blood from Youguiyin were identified.It was revealed that Youguiyin,medicated serum and the main components of Youguiyin absorbed in the blood can promote the proliferation of osteoblasts within a certain concentration range.
