Optimized Process Technology of Wine Steamed Radix Rehmanniae Based on by Multi-index and Response Surface Methodology
10.3870/j.issn.1004-0781.2024.11.022
- VernacularTitle:基于多指标-响应面法优选酒蒸地黄炮制工艺
- Author:
Ruxi GAO
1
,
2
;
Fanyi WANG
;
Xiang LIU
;
Chu YUAN
;
Guoshun SHAN
Author Information
1. 辽宁中医药大学药学院,大连 116600
2. 国家中医药管理局中药炮制工艺原理重点研究室,大连 116600
- Keywords:
Wine steamed Radix Rehmania;
Processing technology;
Mixed weighting;
Response surface method;
Ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS)
- From:
Herald of Medicine
2024;43(11):1833-1843
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the wine steamed Radix Rehmanniae processing technology.Methods The single factor experiment method was used to investigate the wine steamed Rehmannia glutinosa.Ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS)was used to determine the content of active ingredients such as stachyose tetrahydrate,manninotriose,melibiose,fructose,echinacoside,rhmannioside C,rhmannioside D,verbascoside,verbascoside,melittoside,monomelittoside,D(+)-raffinose pentahydrate,8-epicarnolic acid,catalpol,dihydrocatalpol,ajugol and so on.Through analytic hierarchy process and criteria importance through inter-criteria correlation(AHP-CRITIC),the content of the above chemical components and the color and sweetness values of rehmannia decoction pieces were used as evaluation indexes,and the amount of wine,steaming time and stuffiness time of steamed Rehmannia were investigated by a single-factor test method.The Box-Behnken response surface method was used to carry out the experimental design,and the divine Rehmannia processing process optimized.Results The optimal processing technology of wine steamed Radix Rehmanniae was as followed:20.6 g of alcohol per 100 g of herbs,1.5 h of stuffiness,and 6.5 h of steaming time.Conclusion The process of wine steamed rehmannia determined in this experiment is feasible,which can provide a reference for the research of processed rehmannia and provide certain guiding significance for the processed variety in industrial production.
