Effect of Formononetin on Alleviating Inflammatory Injury of Chondrocytes via NF-κB Signaling Pathway
10.3870/j.issn.1004-0781.2024.11.004
- VernacularTitle:芒柄花素调控核因子-κB信号通路缓解软骨细胞炎症损伤作用
- Author:
Fengxiang TIAN
1
;
Peng DING
;
Lingling ZHOU
Author Information
1. 南京中医药大学药学院中药药理教研室,南京 210023
- Keywords:
Formononetin;
Osteoarthritis;
Inflammation;
Nuclear factor-κB signaling pathway
- From:
Herald of Medicine
2024;43(11):1728-1732
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of formononetin on alleviating inflammatory injury of C28/I2 cell,and to explain its mechanism of alleviating inflammatory injury of chondrocytes via NF-κB signaling pathway.Methods The treating concentrations of formononetin were screened,and the model of LPS-induced inflammatory injury in C28/I2 cells was established.Experimental group:normal control group,LPS group,low-dose group(12.5 μmol·L-1),medium-dose group(25 μmol·L-1),and high-dose group(50 μmol·L-1).The cell counting kit 8(CCK-8)was used to detect the effect of formononetin on chondrocyte viability.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of inflammatory cytokine interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the supernatant of C28/I2 cell culture.Western blotting assay was used to measure the protein expressions of nuclear factor kappa B inhibitory protein α(IκBα),phospho-nuclear factor kappa B inhibitory protein α(p-IκBα),nuclear factor kappa B p65(NF-κB p65)and phospho-nuclear factor kappa B p65(p-NF-κB p65)in C28/I2 cells.Results The results of CCK-8 assay showed the chondrocyte viability decreased in the 10 μg·mL-1 LPS group and increased in formononetin group(P<0.01).The results of ELISA experiments showed that the administration of formononetin could reduce the levels of IL-6 and TNF-α in the supernatant of C28/I2 cells(P<0.01 or 0.05)when compared with the LPS group.The results of Western blotting showed that compared with the LPS group,the phosphorylation level of the NFκB pathway could be down-regulated in a dose-dependent way(P<0.01 or 0.05).Conclusion The formononetin can alleviate the inflammatory injury of C28/I2 cell,which is related to the modulation of the phosphorylation activation of NF-κB signal pathway.
