Infrared Fingerprint,TLC Identification and Content Determination of Phenolic Acid Components of Calonyction muricatum(Linn)G.
10.3870/j.issn.1004-0781.2024.10.021
- VernacularTitle:华佗豆的红外指纹图谱和薄层色谱鉴别及酚酸类成分含量测定
- Author:
Jing LIN
1
,
2
;
Jinyu WEI
;
Jie LIANG
;
Yanli LIANG
;
Jiangcun WEI
;
Chunlian LU
;
Piaoxue ZHENG
;
Zhengyi SUN
Author Information
1. 广西中医药大学药学院,南宁 530200
2. 广西农业职业技术大学,南宁 530007
- Keywords:
Calonyction muricatum(Linn)G.;
Infrared fingerprints;
Thin layer chromatography identification;
Phenolic acid components;
Content determination
- From:
Herald of Medicine
2024;43(10):1656-1662
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of infrared fingerprint,TLC identification and content determination of phenolic acid components of Yao medicine Calonyction muricatum(Linn)G.Methods The infrared fingerprint of 10 batches of Calonyction muricatum(Linn)G were established by infrared spectroscopy.The spectral datas were analyzed by similarity analysis,infrared spectroscopy(HCA),principal component analysis(PCA)and Partial-least-squares discriminant analysis(PLS-DA).Chlorogenic acid,heterochlorogenic acid A and caffeic acid of Calonyction muricatum(Linn)G were identified by TLC.The contents of neochlorogenic acid,chlorogenic acid,caffeic acid,cryptochlorogenic acid,isochlorogenic acid A and C were determined simultaneously by HPLC method.Results It could be suggested that organic acids,flavonoids and other compounds of Calonyction muricatum(Linn)G by infrared spectroscopy and nine common peaks were calibrated by infrared fingerprint;the similarity evaluation was above 0.999;the results of cluster analysis(CA)and principal component analysis(PCA)showed that it could be clustered into 2 categories,including S1,S2 and S3 were clustered into one categoriy and the rest were one.5 differential components(VIP>1)were selected by Partial-least-squares discriminant analysis;the test and control samples of TLC showed consistent locations the spots were clear,with good separation degree;the six components of Calonyction muricatum(Linn)G showed good linear relationship(r≥0.999 2),average sample recovery rate 97.77%-102.59%,and RSD less than 2.90% .Conclusion The TLC and infrared fingerprint were simple and stable,and the results of the six components were reliable,which can lay a scientific foundation for the quality control of the materials.
