Screening of Anti-Helicobacter pylori Gastritis Active Component of the Ethyl Acetate Extract of Alpinia officinarum Hance Based on the Knockout Strategy
10.3870/j.issn.1004-0781.2024.09.005
- VernacularTitle:基于"敲除策略"筛选高良姜乙酸乙酯部位抗幽门螺杆菌胃炎活性成分
- Author:
Xin LUO
1
;
Wuyingxiao ZHENG
;
Jianting ZHAN
;
Jingyu YANG
;
Haoran MA
;
Guoping GAN
;
Pengtao YOU
;
Xiaochuan YE
;
Dan LIU
Author Information
1. 湖北中医药大学药学院中药资源与中药化学湖北省重点实验室,武汉 430065
- Keywords:
Alpinia officinarum Hance;
H.pylori gastritis;
Knock out;
Active component;
Active ingredient
- From:
Herald of Medicine
2024;43(9):1387-1392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the anti-Helicobacter pylori gastritis active components of the ethyl acetate extract of Alpinia officinarum Hance.Methods The"knock-out"strategy combined with high-performance liquid chromatography(HPLC)detection was developed to separate the components of the ethyl acetate extract of A.officinarum while obtaining the negative samples without the components.A human gastric epithelial cell(GES-1)model of H.pylori gastritis was established,and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interleukin-8(IL-8)and interleukin-1β(IL-1β)in the supernatant of the cells were determined by enzyme-linked immunosorbent assay(ELISA).Results The total flavonoid fraction,the negative fraction without total diphenylheptanoids,the negative fraction without 5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone(DHPA),and galangin significantly reduced IL-6 levels in the supernatant of H.pylori infected GES-1 cells at a concentration of 8 μg·mL-1 with 24 h incubation.The total flavonoid fraction strongly inhibited the release of IL-6,TNF-α,IL-8,and IL-1β from H.pylori gastritis GES-1 cells at a concentration of 16 μg·mL-1.Conclusions The total flavonoid fraction is the major anti-H.pylori gastritis active component of the ethyl acetate extract of A.officinarum.The results lay the foundation for further elucidation of the material basis of A.officnarum against H.pylori gastritis.
