Research on the Drug Renal Toxicity Biomarkers Based on HK-2 Cells

VernacularTitle:基于HK-2细胞评价药物肾毒性生物标志物的研究
Author: Ruiping XU ^{1
,

2} ; Bingkun XIAO ; Xiaoyao MIAO ; Zhiheng LI ; Rongqing HUANG
Author Information

1. 广东药科大学中药学院,广州 511436
2. 军事科学院军事医学研究院辐射医学研究所,北京 100850
Keywords: Renal toxicity; Evaluation method; Biomarkers; β2-microglobulin(β2-MG)
From: Herald of Medicine 2024;43(8):1187-1191
CountryChina
Language:Chinese
Abstract: Objective Screening for sensitive biomarkers for predicting and analyzing drug-induced renal toxicity,accelerates drug early development.Methods Focuses on epithelial cells of proximal convoluted tubules in human renal cortex(human kidney-2,HK-2)cells as the research object,screening for highly sensitive biomarkers using three nephrotoxic drugs(cisplatin,gentamicin,and aristolochic acid Ⅰ).Results The sensitivity of biomarkers in intracellular fluid is higher than in extracellular fluid,compared to detecting a single biomarker in the intracellular fluid.The combined detection of β2-microglobulin(β2-MG)and neutrophil gelatinase-associated lipocalin(NGAL)improved the accuracy of renal toxicity evaluation.Conclusion Based on the HK-2 cell model,the combined detection of β2-MG and NGAL in intracellular fluid can be used to predict renal toxicity in the drug's early development stage.