Determination of 11 Components in Fazhi Banxia Qu by QAMS and Quality Evaluation of Theirs GRA and EW-TOPSIS
10.3870/j.issn.1004-0781.2024.07.017
- VernacularTitle:一测多评法测定法制半夏曲中11种成分含量及其GRA、EW-TOPSIS质量评价
- Author:
Bo SHU
1
;
Guoping LEI
;
Bin YUAN
Author Information
1. 川北医学院附属医院药剂科,南充 637000
- Keywords:
Fazhi Banxia Qu;
Quantitative analysis of multi-components by single-marker;
Multi-index components;
Relative correction factors;
Grey relational analysis;
Entropy weight-technique for sequencing by approximate ideal solution or technique for order prefer
- From:
Herald of Medicine
2024;43(7):1120-1126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous determination of 11 components in Fazhi Banxia Qu(including Pinelliae Preparata,Fritillariae Cirrhosae Bulbus,and Exocarpium Citri Grandis),and combined with grey relational analysis(GRA)and entropy weight-technique for sequencing by approximate ideal solution or technique for order preference by similarity to ideal solution(EW-TOPSIS)analysis to comprehensive evaluate the quality of Fazhi Banxia Qu.Methods The Shimadzu C18 column was used,and the mobile phase consisted of acetonitrile-0.5%glacial acetic acid in a gradient elution manner,the flow rate was 1.0 mL·min-1,and the detection wavelength were set at 254 nm and 290 nm.Ethyl-p-methoxycinnamate was used as an internal reference substance to calculate the other ten components'relative correction factors(RCF),after which the content determination was made.The GRA combined with the EW-TOPSIS model were used to evaluate the comprehensive quality of Fazhi Banxia Qu.Results Eleven components of Fazhi Banxia Qu showed good linear relationships within their ranges,and the correlation coefficients were greater than 0.999.The average recoveries were 96.94%-100.12%(RSD<2.0%,n=9).There was no significant difference between QAMS and external standard method(ESM).The relative correlation degree of the GRA model was 0.290 3-0.618 7,and the relative proximity in the EW-TOPSIS model was 0.211 4-0.634 3.The comprehensive evaluation results of the GRA and EW-TOPSIS models were the same.Conclusion The HPLC-QAMS method is convenient and accurate,and can be used for quantitative control of multi-index components in Fazhi Banxia Qu.The GRA combined with EW-TOPSIS models can be used to evaluate the comprehensive quality of Fazhi Banxia Qu.
