Evaluation of the Difference Between Angelica-Astragalus Medicine Pair Dispensing Granules Decoction and Traditional Decoction Based on Chemical Composition and Pharmacological Action
10.3870/j.issn.1004-0781.2024.07.016
- VernacularTitle:基于化学成分与药理作用评价当归-黄芪药对配方颗粒汤剂与传统汤剂的差异
- Author:
Jing YAO
1
,
2
,
3
,
4
;
Lijie MA
;
Yanna REN
;
Xiaopeng LI
;
Junhan SHI
;
Xinjing GUI
;
Lu ZHANG
;
Qingxiao WANG
;
Xuelin LI
;
Ruixin LIU
Author Information
1. 河南中医药大学第一附属医院药学部,郑州 450000
2. 河南省中药临床应用、评价与转化工程研究中心,郑州 450000
3. 河南省中药临床药学中医药重点实验室,郑州 450000
4. 河南中医药大学呼吸疾病中医药防治省部共建协同创新中心,郑州 450046
- Keywords:
Angelica-astragalus medicine pair;
Danggui Buxue decoction;
Traditional decotion;
Dispensing granules
- From:
Herald of Medicine
2024;43(7):1112-1119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the differences in chemical composition and pharmacological effects between Angelica-Astragalus medicine pair decoction(DGD)and traditional decotion,and to provide a reference for the rational clinical application of Danggui Buxue decoction.Methods With the two comparison methods of unified and non-uniform raw material source batches,we set up different sample groups,established characteristic maps by HPLC,and evaluated the chemical components based on the similarity of characteristic maps,component types,index component content,common peak area,and other factors.The efficacy of the drug was evaluated in the hemorrhagic blood deficiency model mice.Results ①The similarity of the feature map between the DGD and TD was high(similarity was greater than 0.87).②The number of chromatographic peaks was inconsistent.Traditional decoction from self-purchased decoction pieces,or traditional decoction-Factory A decoction pieces had a total of 12 chromatographic peaks each.The DGD of Factory A had a total of 15 chromatographic peaks.There were 10 chromatographic peaks in the DGD of Factory B.③The contents of ferulic acid and calycosin 7-O-glucoside(CG)in DGD of Factory A were higher than those in traditional decoction(P<0.05,n=3).There was no significant difference between DGD and TD ferulic acid content in Factory B,but the content of CG was lower than that in traditional decoction(P<0.05).④The total area of common peaks in DGD was different from that in TD.The relative total ratios of the contents of the components in the self-purchased traditional decoction pieces,the traditional decoction pieces of Factory A,the formula granules of Factory A,and the formula granules of Factory B were 1.00,0.96,2.14,0.60,respectively.⑤Both DGD and traditional decoction could significantly promote the recovery of hemoglobin and red blood cells in hemorrhagic anemia model mice(P<0.01);Compared with the model control group,there was a significantly difference(P<0.05)except for the DGD group of Plant B.There was no significant difference between DGD and TD of Plant A,but there was a very significant difference between DGD and TD of Plant B(P<0.01).Conclusion Whether the raw material source batch is consistent or not,DGD and TD have certain differences in chemical composition.In terms of pharmacological effect,DGD,prepared from a unified batch of decoction pieces,has similar efficacy to traditional decoction in alleviating hemorrhagic anemia.There are certain differences in the pharmacological effects between DGD prepared from different batches of decoction pieces and traditional decoctions.The differences caused by the different preparation processes of the same source batch of prepared slices were compared,and the quality differences of the formula granules from different manufacturers were caused by the different source batches of prepared slices and different preparation processes,indicating the necessity and urgency of the country to formulate a unified quality standard for formula granules and related process specifications.
