Effect and Molecular Mechanism of Paeonol on Renal Interstitial Fibrosis in Rats
10.3870/j.issn.1004-0781.2024.07.005
- VernacularTitle:丹皮酚对大鼠肾间质纤维化的影响及其分子机制
- Author:
Haiyang GAO
1
;
Jincun ZHANG
;
Xi CHEN
;
Xiaohai GUAN
;
Fenghong CAO
;
Shaosan KANG
;
Weixing GAO
;
Lei WANG
;
Anliang YAO
;
Jian LIU
;
Liguo ZHANG
Author Information
1. 华北理工大学附属医院泌尿外科,唐山 063000
- Keywords:
Paeonol;
Renal interstitial fibrosis;
Unilateral ureteral obstruction;
SIRT1/Nrf2 signaling pathway
- From:
Herald of Medicine
2024;43(7):1055-1060
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of paeonol(PAE)on renal interstitial fibrosis in rats.Methods The rats were randomly divided into sham operation(Sham)group,unilateral ureteral obstruction(UUO)group,PAE low dose(PAE-L)group,PAE medium dose(PAE-M)group,PAE high dose(PAE-H)group and irbesartan(IRB)group.Except for the Sham group,the UUO model was established in other groups.Each group was given a corresponding intervention for two weeks.Serum creatinine(Scr),blood urea nitrogen(BUN),serum 8-hydroxydeoxyguanosine(8-OHdG)levels,renal tissue superoxide dismutase(SOD),glutathione peroxidase(GPX)activities,α-smooth muscle actin(α-SMA),type Ⅰ collagen(Col-Ⅰ),fibronectin(FN),silent information regulator 1(SIRT1),nuclear factor E2-related factor 2(Nrf2)protein expression were detected;observe pathological changes of kidney tissue and calculate collagen volume fraction(CVF).Results Compared with the UUO group,the serum levels of Scr,BUN,and 8-OHdG in each dose group of PAE were decreased,the activities of SOD and GPX in kidney tissue were increased,the positive expressions of α-SMA,Col-Ⅰ and FN in kidney tissue were decreased,and the protein expressions of SIRT1 and Nrf2 were increased.Masson staining showed a decrease of CVF in renal tissue(all P<0.05),and HE staining showed a different degree of improvement in pathological changes such as inflammatory cell infiltration and tubular dilatation in renal tissue;PAE improves renal interstitial fibrosis in rats in a dose-dependent manner(P<0.05),and the effect of large dose PAE on renal interstitial fibrosis in rats was similar to that of IRB.Conclusion PAE can alleviate UUO-induced rat renal interstitial fibrosis and oxidative stress,and improve rat renal function.And this mechanism may be related to the activation of the SIRT1/Nrf2 signaling pathway.
