Enrichment of Crude Alkaloids from Zanthoxylum nitidum
10.3870/j.issn.1004-0781.2024.07.004
- VernacularTitle:两面针粗生物碱的富集
- Author:
Pu ZHENG
1
,
2
,
3
;
Xinglian XU
;
Lulu DENG
;
Jiang LI
;
Yanhua FAN
;
Shuzhen MU
Author Information
1. 贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵阳 550014
2. 贵州省中国科学院天然产物化学重点实验室,贵阳 550014
3. 贵州医科大学药学院,贵阳 550025
- Keywords:
Zanthoxylum nitidum(Roxb.)DC;
Crude alkaloids;
Extraction and enrichment;
Macroporous adsorption resin;
Anti-leukemia
- From:
Herald of Medicine
2024;43(7):1050-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the enrichment method of the crude alkaloid of Zanthoxylum nitidum(Roxb.)DC.(Z.nitidum),which can inhibit the proliferative activity of human erythroid leukemia cells(HEL cells)in vitro,and to provide a scientific basis for the antitumor activity of Z.nitidum.Methods Z.nitidum was extracted using different concentrations of ethanol(60%,70%,80%,90%,and 100%)to determine the optimum extraction conditions according to the highest extraction rate.Static and dynamic adsorption determined the adsorption capacity of different macroporous resins(HPD 100,XDA-5,D101,HPD 722,HPD 400)on the ethanol extracts from Z.nitidum.The effects of crude alkaloids on the proliferation activity of HEL cells in each group were studied by the MTT method.Results Z.nitidum was extracted from 60%,70%,80%,90%,and 100%ethanol,and the extract with 60%ethanol concentration had the highest yield(11.937±0.002)%.Static and dynamic adsorption experiments showed that the adsorption capacity of the above five kinds of macroporous resins was 1∶500(extract of Z.nitidum∶macroporous resin).The crude alkaloids enriched by different macroporous resins have different effects on the proliferation activity of HEL cells.The inhibition rates(%)at a concentration of 80 μg·mL-1 of Z.nitidum were(59.62±3.46)%(XDA-5),(55.81±5.16)%(HPD 100),(59.33±1.33)%(D101),(79.63±1.02)%(HPD 722),and(83.97±0.38)%(HPD 400),respectively.HPD 400 macroporous resin-enriched crude alkaloids inhibited the proliferation of HEL cells most significantly(P<0.01).Conclusion The process of enriching the crude alkaloids of Z.nitidum using macroporous resin was simple and feasible,and the crude alkaloids enriched by HPD 400 showed significant inhibitory effects on HEL cells with good anti-leukemic activity.
