Effect of Aqueous Extract of Scrophularia ningpoensis on AMPK Activity in INS-1 Cells under High Glucose Exposure
10.3870/j.issn.1004-0781.2024.06.002
- VernacularTitle:玄参水提取物对高糖暴露条件下INS-1细胞AMPK的激活作用
- Author:
Xu GUO
1
,
2
,
3
;
Jun ZHOU
;
Xiaohan LI
;
Shiqi CHEN
;
Yanguo GAO
;
Yonghong ZHANG
;
Qibin WANG
;
Tao ZHENG
;
Li CHEN
Author Information
1. 湖北医药学院药学院,十堰 442000
2. 湖北医药学院附属太和医院武当中医药研究所,十堰 442000
3. 湖北医药学院附属太和医院药学部,十堰 442000
- Keywords:
Scrophularia ningpoensis;
AMP-activated protein kinase;
High glucose exposure;
INS-1 cells;
Fluorescence resonance energy transfer
- From:
Herald of Medicine
2024;43(6):850-854
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aqueous extract of Scrophularia ningpoensis(AESN)on AMP-activated protein kinase(AMPK)activity in INS-1 cells under high glucose(HG)conditions.Methods INS-1 cells were cultured in HG medium and treated with different concentrations of AESN.The influences of AESN on cell viability and pyroptotic body formation were detected using the CCK-8 assay.Western blotting was used to observe the effects of AESN on AMPK expression and phosphorylation.The effect of AESN on AMPK activity was measured using time-resolved fluorescence resonance energy transfer(TR-FRET)assay.Results Compared with normal glucose controls,INS-1 cells exposed to HG showed decreased cell viabilities and increased formation of pyroptotic bubbles using CCK-8 assay.Western blotting results indicated that HG exposure led to a decrease in intracellular AMPK phosphorylation levels.However,co-incubation with AESN under HG exposure AESN treatment increased the cell viabilities and phosphorylation of AMPK and decreased the pyroptotic bubbles formation in a concentration-dependent manner.In addition,the results of TR-FRET revealed that AESN treatment effectively increased AMPK activity in a concentration-dependent manner.Conclusion AESN has an activating effect on AMPK activity in HG-treated INS-1 cells.
