Mechanism of Piezo1-induced Actin Cytoskeleton Remodeling in Promoting the Metastasis of Cervical Cancer
10.11969/j.issn.1673-548X.2024.10.014
- VernacularTitle:Piezo1通过驱动肌动蛋白细胞骨架重塑促进宫颈癌侵袭迁移的机制研究
- Author:
Juexiao DENG
1
;
Yang LI
;
Meng GONG
Author Information
1. 430060 武汉大学人民医院妇产科
- Keywords:
Cervical cancer;
Piezo1;
Actin cytoskeleton;
Invasion;
Migration
- From:
Journal of Medical Research
2024;53(10):74-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and mechanism of Piezo1 in the invasion and migration of cervical cancer by driving actin cytoskeletal remodeling.Methods Immunohistochemistry and Western blot were used to detect the expression of Piezo1 and F-ac-tin in cervical cancer tissues and cell lines;lentiviral transfection resulted in silencing of the Piezo1 gene in cervical cancer cell lines Siha and Hela,and the transwell assay was used to detect the effect of Piezo1 on the invasion and migration of cervical cancer.Phalloidin stai-ning was used to observe the remodeling effect of Piezo1 on the actin cytoskeleton,and the mechanism of Piezo1 affecting the invasion and migration of cervical cancer was further confirmed by intervening in the cytoskeletal polymerization state.Results The expression levels of Piezo1 and F-actin in the cervical cancer group were significantly higher than those in the control group,and the expressions of Piezo1 and F-actin in the cervical cancer group were higher than that in the cervical cancer group without metastasis.Silencing Piezo1 downreg-ulated the expressions of F-actin in SiHa and HeLa cell lines and inhibited cervical cancer invasion and migration,which was partially alleviated by the actin polymerization inducer Jasplakinolide,while the activation of Piezo1 upregulated F-actin expression and promoted the invasion and migration of cervical cancer,which could be inhibited by actin depolymerizer Latrunculin A.Conclusion Piezo1 is highly expressed in cervical cancer and drives actin cytoskeletal remodeling to promote the invasion and migration of cervical cancer.
