Bioinformatics-based Analysis of the Relationship between Osteoporosis and Chronic Obstructive Pulmonary Disease
10.11969/j.issn.1673-548X.2024.10.012
- VernacularTitle:基于生物信息学分析骨质疏松症与慢性阻塞性肺疾病的相关性
- Author:
Yifu YANG
1
;
Shuhua LIU
;
Tongying CHEN
Author Information
1. 510405 广州中医药大学第三临床医学院
- Keywords:
Osteoporosis;
Chronic obstructive pulmonary disease;
Bioinformatics;
Microribonucleic acid
- From:
Journal of Medical Research
2024;53(10):60-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze differentially expressed microRNAs(DEmiRNAs)in osteoporosis(OP)and chronic obstructive pulmonary disease(COPD)using bioinformatics tools,and to explore the interrelationship between these two diseases.Methods Gene expression microarrays for OP and COPD were retrieved from the GEO database.Differential analysis was conducted using the limma pack-age in R software version 4.1.0,identifying DEmiRNAs between OP and COPD.The selected DEmiRNAs were then subjected to target gene prediction using the miRDB and TargetScan databases.Predicted target genes were analyzed for transcription factor predictions using KOBAS,followed by GO and KEGG pathway analyses.Protein-protein interaction(PPI)network data for the target genes were down-loaded from the STRING database and analyzed and visualized using Cytoscape to construct a PPI network and model.Results Four DEmiRNAs were identified as differentially expressed between OP and COPD microarrays:hsa-miR-631,hsa-miR-940,hsa-miR-508-5p and hsa-miR-1470.PPI network analysis revealed seven core genes:UBA52,UBE2I,UBE2N,STAM,IPO5,CD28 and STX6.Conclusion The interconnection between OP and COPD may be mediated through a series of physiological and pathological responses such as hypoxia,chronic inflammation,oxidative stress,calcium ion reabsorption,and mitochondrial autophagy.Of these,hsa-miR-940 exhibits the closest relationship between the two diseases,suggesting a pivotal role in linking their pathogenesis.
