Study on the Hepatoprotective Effect and Key Molecule Docking of Piperlongumine in Inhibiting Hepatic Stellate Cell Pyroptosis
10.11969/j.issn.1673-548X.2024.10.009
- VernacularTitle:荜茇酰胺抑制肝星状细胞焦亡发挥肝保护作用及关键分子对接研究
- Author:
Li ZHAO
1
;
Ziqi GUO
;
Yong YANG
Author Information
1. 010110 呼和浩特,内蒙古医科大学基础医学院
- Keywords:
Piperlongumine;
Pyroptosis;
Hepatic stellate cells;
Molecular docking;
GSDMD
- From:
Journal of Medical Research
2024;53(10):39-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective Exploring the protective effect and mechanism of piperlongumine(PL),an active ingredient in the extract of Piper longum,on hepatic stellate cell damage in vitro.Methods The liver stellate cells(HSC-T6)were activated by adding lipopo-lysaccharide(LPS),and the liver stellate cells were treated with different concentrations of piperlongumine,and the protein and mRNA expression levels of IL-18,GSDMD,NLRP3 and caspase-1 in the cells were detected by Western blot and qPCR.Immunofluorescence staining showed the localization and expression of IL-18,GSDMD,NLRP3 and caspase-1 in cells.Molecular docking technology was used to analyze the target molecules of the action of piperlongumine.Results In vitro experiments showed that piperlongumine could in-hibit the protein and mRNA expression of IL-18,GSDMD,NLRP3 and caspase-1 in a concentration-dependent manner.The results of molecular docking verified the correlation,and piperlongumine could bind to IL-18,GSDMD and caspase-1,but had a poor binding effect with NLRP3.Conclusion Piperlongumine can prevent pyroptosis by inhibiting the caspase-1 signaling pathway of HSC-T6 cells,thereby exerting a protective effect on liver injury.
