IgM Subclass Antibody 3A6 Inhibits Atherosclerotic Plaque Formation in ApoE-/-Mice
10.11969/j.issn.1673-548X.2024.09.022
- VernacularTitle:IgM亚类抗体3A6抑制ApoE-/-小鼠动脉粥样硬化斑块形成
- Author:
Hui DAI
1
;
Xiaofei SHI
;
Kaikai HAO
Author Information
1. 744000 平凉,甘肃医学院临床医学系
- Keywords:
Natural antibody;
3A6;
Oxidized low density lipoprotein;
Atherosclerosis;
ApoE-/-mice
- From:
Journal of Medical Research
2024;53(9):113-116,195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the anti-oxidized low density lipoprotein(ox-LDL)IgM subclass antibody 3A6 on the atherosclerotic plaque formation in ApoE-/-mice.Methods The ApoE-/-mice were randomly divided into three groups:control group(intraperitoneal injection of normal saline),5G8group(intraperitoneal injection of nature anti-LDL IgM subclass antibody)and 3A6group(intraperitoneal injection of natural anti-ox-LDL IgM subclass antibody),with 6mice in each group.The body weight of mice and the level of serum lipid in each group were measured after 12 weeks of high-fat and high-cholesterol diet.hematoxylin-eosin staining was used to analyze the pathological changes and plaque area of the aorta in mice.Results There was no significant difference in body weight,serum lipid level and ox-LDL level among the three groups(P=0.087).Compared with the control group,the area of atherosclerotic plaque was significantly decreased in the 3A6group(P=0.023).Conclusion The natural IgM subclass antibody 3A6 can effectively inhibit the formation of atherosclerotic plaque in ApoE-/-mice.
