Effect of FOXO3a Targeting on Alternative Splicing of Vascular Endothelial Cell Related Genes
10.11969/j.issn.1673-548X.2024.09.013
- VernacularTitle:FOXO3a靶向调控血管内皮细胞相关基因可变剪接的作用研究
- Author:
Zhuo CHEN
1
;
Ying GAO
;
Yu WANG
Author Information
1. 830011 乌鲁木齐,新疆医科大学第二临床医学院
- Keywords:
Coronary heart disease;
FOXO3a;
Targeted regulation;
Alternative splicing
- From:
Journal of Medical Research
2024;53(9):62-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of vascular endothelial cells in coronary artery disease,focusing on the role of forkhead box O3a(FOXO3a)in regulating target gene expression and alternative splicing in vascular endothelial cell injury.Methods Transcriptome data was obtained by overexpressing FOXO3a in human vascular endothelial cell lines using transcriptome techniques.The expression lev-els and alternative splicing patterns of potential target genes regulated by FOXO3a were analyzed,as well as the functions of these genes.Results In HUVEC cells overexpressing FOXO3a,419genes were differentially expressed compared with the control group.The results of the geneontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)analysis showed that up-regulated genes were en-riched in the inflammatory signaling pathway,while down-regulated genes were enriched in the metabolic pathway.Based on transcrip-tome data,alternative splicing analysis revealed significant differences in the patterns of 1784 alternative splicing events between the FOXO3a overexpression group and the control group.The results of the GO and KEGG analysis showed that differentially variable splicing genes were enriched in apoptosis-related pathways.Conclusion FOXO3a can affect the apoptosis of vascular endothelial cell by regula-ting the expression and alternative splicing of genes related to immune inflammation,lipid metabolism,and cell apoptosis,which may af-fect the occurrence of coronary heart disease.
