Bioinformatics Analysis of miRNA/mRNA Network Regulated by Long Non-coding RNA PURPL
10.11969/j.issn.1673-548X.2024.07.026
- VernacularTitle:长链非编码RNA PURPL调控miRNA/mRNA网络的生物信息学分析
- Author:
Xueying GUO
1
;
Ruitao ZHANG
;
Tingting HE
Author Information
1. 450052 郑州大学第一附属医院妇科
- Keywords:
Bioinformatics;
Ovarian cancer;
Non-coding RNA;
PURPL
- From:
Journal of Medical Research
2024;53(7):129-135,140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen microRNA(miRNA)that could bind to long non-coding RNA(lncRNA)p53 upregulated regu-lator of p53 levels(PURPL),and downstream target genes and signaling pathways by comprehensive bioinformatics database mining,con-struct PURPL/miRNA/target genes/signaling pathways regulatory network,and provide foundation for further study on the molecular mechanism of PURPL involved in the invasion and metastasis of ovarian cancer cells.Methods Based on the lncRNA/miRNA regulatory mechanism,using multi-database to cross-predict the downstream miRNA that could bind to PURPL,and target genes for miRNA.Protein-protein interaction(PPI)network analysis and core protein screening for target gene transcription proteins were conducted.Fi-nally,core genes were analyzed by gene ontology(GO),disease and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling path-ways enrichment analysis.Results PURPL were differentially expressed in normal tissues and cancer tissues(including normal ovarian tissues and ovarian cancer tissues).The 3 downstream miRNA and 341 target genes were screened,and 15 core genes were screened out through PPI network analysis and enrichment analysis.Finally,the regulatory network composed of PURPL,3 downstream miRNA,15 core target genes and potential signaling pathways was determined.Conclusion PURPL/miRNA/target genes/signaling pathways regula-tory network might provide potential molecular targets for the subsequent molecular mechanism research for the invasion and metastasis of ovarian cancer.
