Correlation Analysis of Gasdermin E and Cervical Cancer
10.11969/j.issn.1673-548X.2024.07.009
- VernacularTitle:焦孔素蛋白E与宫颈癌相关性分析
- Author:
Jiani CHEN
1
;
Chengze LIN
;
Jiani YANG
Author Information
1. 325035 温州医科大学基础医学院
- Keywords:
Gasdermin E;
Cervical cancer;
Correlation
- From:
Journal of Medical Research
2024;53(7):38-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the correlation between gasdermin E(GSDME)and the occurrence and development of cervical cancer.Methods HPA database and GEPIA2 online website were used to analyze the differential expression of GSDME gene and protein between cervical cancer tissues and normal cervical tissues.GEPIA2 and KM plotter online website was used to analyze the correlation be-tween GSDME gene expression and the pathological staging,survival and prognosis of cervical cancer.cBioPortal online website was used to analyze the GSDME gene mutation in cervical cancer tissues.TIMER2 online website and R-package were used to analyze the correla-tion between GSDME and immune infiltration in cervical cancer tissues.Gene set enrichment analysis was used to analyze the biological function of GSDME in development of cervical cancer.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to verify the transcription and translation of GSDME in cervical cancer cell lines.Results Compared with normal cervical tis-sues,the expression of GSDME mRNA and protein in cervical cancer tissues was lower(P＜0.05).Survival analysis showed that the high expression of GSDME gene may be associated with poor prognosis of cervical cancer patients.0.36％of GSDME amplification muta-tion was found in cervical squamous cell carcinoma.There was significant negative correlation between high expression of GSDME gene and B lymphocyte infiltration,whereas a significant positive correlation between high GSDME gene and mast cell infiltration in cervical cancer tissues(P＜0.05).The high expression of GSDME in cervical cancer exhibited a significant negative correlation with glutathione peroxidase activity,mRNA modification,keratin filament,and other biological functions(P＜0.001).RT-qPCR and Western blot con-firmed that the transcription and protein expression of GSDME could be detected in both human papilloma virus(HPV)positive and nega-tive cervical cancer cells.Conclusion GSDME may be a risk factor related to cervical cancer,and the correlation analysis between GS-DME and cervical cancer provides a new perspective for the follow-up targeted treatment of cervical cancer based on GSDME.
