Low-expression of ACOT4 Promotes the Damage and Crystals Formation of HK2 Cell by Calcium Oxalate

VernacularTitle:低表达ACOT4促进草酸钙对HK2细胞损伤和结晶形成
Author: Shenghan WANG ¹ ; Zhentao LEI ; Yuqiang SHI
Author Information

1. 100049 北京,航天中心医院泌尿外科
Keywords: Kidney stones; Calcium oxalate; ACOT4; HK2 cell
From: Journal of Medical Research 2024;53(6):113-118
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of ACOT4 expression on the formation of calcium oxalate stones.Methods The HK2 cell of human tubular epithelial cells was used as subject,HK2 cell was treated with calcium oxalate,and the expression of ACOT4 was interfered with by siRNA.The gene expression levels in HK2 cell were detected by qPCR and Western blot.The cell viability was de-tected by CCK-8 assay.The cell apoptosis was detected by flow cytometry.The cell damage was detected by LDH assay.The adhesion ability of HK2 cell to calcium oxalate crystals was detected by crystal adhesion experiment.Results Calcium oxalate could regulate the expression of ACOT4 in HK2 cell.Interfering with ACOT4 can significantly inhibit the proliferation ability of HK2 cell,and promote the effect of cell activity reduction,damage and apoptosis of calcium oxalate to HK2 cell.At the same time,interfering with ACOT4 can sig-nificantly promote the adhesion ability of HK2 cells to calcium oxalate crystals.Conclusion Knocking down of ACOT4 can promote the damage of calcium oxalate to HK2 cell and promote the adhesion ability of HK2 cell to calcium oxalate crystals.