Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes.
- Author:
Seok Hyun KIM
1
;
Sung Mi CHOI
;
Byung Chul JEE
;
Hee Sun KIM
;
Sun Kyung OH
;
Chang Suk SUH
;
Young Min CHOI
;
Jung Gu KIM
;
Jin Yong LEE
;
Shin Yong MOON
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Mouse oocytes;
Cryopreservation;
DMSO;
Slow freezing and ultrarapid thawing;
Meiotic spindle
- MeSH:
Animals;
Cryopreservation;
Dimethyl Sulfoxide;
Freezing;
Incidence;
Mice*;
Oocytes*;
Spindle Apparatus*;
Tubulin
- From:Korean Journal of Obstetrics and Gynecology
2001;44(9):1602-1608
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes. METHODS: After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated. RESULTS: The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group. CONCLUSION: The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.
