Signal interference between drugs and metabolites in LC-ESI-MS quantitative analysis and its evaluation strategy
10.1016/j.jpha.2024.02.008
- Author:
Jiang FULIN
1
,
2
;
Liu JINGYU
;
Li YAGANG
;
Lu ZIHAN
;
Liu QIAN
;
Xing YUNHUI
;
Zhu JANSHON
;
Huang MIN
;
Zhong GUOPING
Author Information
1. Institute of Clinical Pharmacology,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou,510080,China
2. Guangdong RangerBio Technologies Co.,Ltd.,Dongguan,Guangdong,523000,China
- Keywords:
Liquid chromatography-electrospray;
ionization tandem mass spectrometry(LC-ESI-MS);
Drugs;
Metabolites;
Ionization interference;
Quantitative analysis
- From:
Journal of Pharmaceutical Analysis
2024;14(7):1024-1034
- CountryChina
- Language:Chinese
-
Abstract:
Liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS)is a widely utilized technique for in vivo pharmaceutical analysis.Ionization interference within electrospray ion source,occurring between drugs and metabolites,can lead to signal variations,potentially compromising quantitative accuracy.Currently,method validation often overlooks this type of signal interference,which may result in systematic errors in quantitative results without matrix-matched calibration.In this study,we conducted an investigation using ten different groups of drugs and their corresponding me-tabolites across three LC-ESI-MS systems to assess the prevalence of signal interference.Such in-terferences can potentially cause or enhance nonlinearity in the calibration curves of drugs and metabolites,thereby altering the relationship between analyte response and concentration for quanti-fication.Finally,we established an evaluation scheme through a step-by-step dilution assay and employed three resolution methods:chromatographic separation,dilution,and stable labeled isotope internal standards correction.The above strategies were integrated into the method establishment process to improve quantitative accuracy.
