On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay
10.1016/j.jpha.2023.09.011
- Author:
Gao JUNXIA
1
;
Zhang TIANYI
;
Fang YIHUA
;
Zhao YING
;
Yang MEI
;
Zhao LI
;
Li YE
;
Huang JUN
;
Zhu GUONIAN
;
Guo YIRONG
Author Information
1. Institute of Pesticide and Environmental Toxicology,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Hangzhou,310058,China
- Keywords:
Lateral flow immunoassay;
Rapid detection;
Pesticide multi-residue;
Tea matrix;
Sample rapid pretreatment
- From:
Journal of Pharmaceutical Analysis
2024;14(2):276-283
- CountryChina
- Language:Chinese
-
Abstract:
The application of pesticides(mostly insecticides and fungicides)during the tea-planting process will undoubtedly increase the dietary risk associated with drinking tea.Thus,it is necessary to ascertain whether pesticide residues in tea products exceed the maximum residue limits.However,the complex matrices present in tea samples comprise a major challenge in the analytical detection of pesticide residues.In this study,nine types of lateral flow immunochromatographic strips(LFICSs)were developed to detect the pesticides of interest(fenpropathrin,chlorpyrifos,imidacloprid,thiamethoxam,acet-amiprid,carbendazim,chlorothalonil,pyraclostrobin,and iprodione).To reduce the interference of tea substrates on the assay sensitivity,the pretreatment conditions for tea samples,including the extraction solvent,extraction time,and purification agent,were optimized for the simultaneous detection of these pesticides.The entire testing procedure(including pretreatment and detection)could be completed within 30 min.The detected results of authentic tea samples were confirmed by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),which suggest that the LFICS coupled with sample rapid pretreatment can be used for on-site rapid screening of the target pesticide in tea products prior to their market release.
