Design of intelligent horizontal rotating cell culture device
10.19745/j.1003-8868.2024171
- VernacularTitle:智能水平旋转式细胞培养装置设计
- Author:
Li-Fei YANG
1
;
Ye-Rong QIAN
;
Jun-Xi XIANG
;
Ai-Hua SHI
;
Xin LIU
;
Sha-Sha WEI
;
Yi LYU
;
Peng LIU
Author Information
1. 西安交通大学第一附属医院陕西省再生医学与外科工程研究中心,西安 710061
- Keywords:
rotating culture;
cell culture;
cell culture device;
tubular electrospun scaffold;
artificial bile duct
- From:
Chinese Medical Equipment Journal
2024;45(9):41-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an intelligent horizontal rotating cell culture device with high modularity,easy operation,easy disinfection,low cost and high stability.Methods The cell culture device consisted of a rotating culture module,a dirve module,a control module and control software,with the shells of all the modules being manufactured by 3D printing.The rotating culture module was composed of a tubular electrospun scaffold,a cell culture chamber,a magnetic coupling rotor and polypropylene pipeline;the drive module was made up of a N20 reduction motor and a magnetic coupling rotor;the control module included an ESP-8266 chip and a printed circuit board;the control software was developed with Blinker IoT platform and C++language.The device was used to culture human intrahepatic bile duct epithelial cells to verify its effects.Results Light microscopy and scanning electron microscopy images showed that a uniform and continuous cell layer was formed on the surface of the tubular electrospun scaffold.Conclusion The intelligent horizontal rotating cell culture device achieves uniform growth of cells on the inner surface of tubular electrospun scaffolds,and can be used as an effective platform for cell culture on tubular scaffolds.[Chinese Medical Equipment Journal,2024,45(9):41-45]
